Mirus Bio – Transforming the Science of Transfection

Key Features & Benefits

• High Transfection Efficiency
  Optimized for effective DNA delivery, helping maximize viral vector yields and supporting consistent high-titer AAV and lentivirus production. 

• Scalable Workflow
  Delivers reproducible performance across a broad range of culture volumes, making it suitable for both early-stage research and larger production campaigns. 

• Broad Compatibility
  Works efficiently with adherent and suspension HEK293 cells and is compatible with multiple recombinant virus production and cell culture systems.

• Streamlined Virus Generation
  Simplifies transfection workflows, reducing process complexity while maintaining reliable virus production performance.

• Animal-Origin Free Formulation
  Manufactured using a fully synthetic formulation, supporting modern research and bioprocessing requirements.

Ideal for Research and Bioprocess Development

TransIT-VirusGEN® is widely used for viral vector generation in academic research, gene therapy development, and biopharmaceutical discovery programs. For users seeking even greater viral yields, performance can be further enhanced using the VirusGEN® AAV Transfection Kit or VirusGEN® LV Transfection Kit, which include optimized enhancer technologies designed to increase vector production.

For large-scale and regulated manufacturing applications, TransIT-VirusGEN® GMP provides a suitable solution to support viral vector production workflows requiring GMP-grade materials.

Key Benefits

• High Transfection Efficiency
  Achieve effective delivery of plasmid DNA, RNA, CRISPR components, and other cargo into difficult-to-transfect cells, including primary and stem cell populations.

• Broad Device Compatibility
  Designed for use with many commonly available electroporation systems, including the Ingenio® EZporator®, Lonza Nucleofector®, Bio-Rad®, and Harvard BTX® platforms.

• Cost-Effective Alternative
  Researchers can reduce transfection costs while maintaining strong performance, making Ingenio® an attractive alternative to more expensive proprietary electroporation kits.

• Extended Shelf Life
  A one-year shelf life helps minimize waste and ensures reagents remain available for long-term projects and routine laboratory use.

• Flexible Purchasing Options
  Available as standalone electroporation solution or as convenient kits that include cuvettes and accessories, allowing laboratories to purchase only the components they need.

Optimized for a Wide Range of Applications

Ingenio® Electroporation Solutions support efficient plasmid DNA delivery across numerous cell types and have been successfully used with established electroporation systems to achieve high transfection efficiencies while preserving cell health.

The platform is also well suited for modern genome engineering workflows, including CRISPR/Cas9 ribonucleoprotein (RNP) delivery, enabling researchers to perform gene-editing experiments efficiently in suspension and difficult-to-transfect cell lines.

Performance, Flexibility, and Value

Compared with many traditional electroporation kits, Ingenio® solutions provide a combination of strong performance, extended reagent stability, and significant cost savings. This makes them a practical choice for laboratories seeking scalable and economical transfection workflows without compromising experimental results.

Ideal for Research Applications

Whether working with stem cells, immune cells, primary cells, or established cell lines, Ingenio® Electroporation Solutions offer a versatile platform for:

• Plasmid DNA transfection
• mRNA and RNA delivery
• CRISPR/Cas9 genome editing workflows
• Gene expression studies
• Cell engineering and therapeutic research
• Stem cell and primary cell applications

Researchers worldwide rely on Ingenio® technology for consistent electroporation performance, making it a trusted solution for both routine laboratory transfections and advanced cell engineering studies.

Key Benefits

• Broad Cell Type Compatibility
  Efficient delivery across a wide range of mammalian cell types, including primary cells, stem cells, adherent cultures, and suspension cells.

• High Transfection Efficiency
  Optimized formulations promote strong gene expression and effective nucleic acid delivery, helping researchers achieve consistent experimental results. 

• Low Cellular Toxicity
  Many TransIT® reagents are designed to balance high delivery performance with excellent cell viability, reducing experimental bias and improving downstream analyses.

• Flexible Application Support
  Suitable for plasmid DNA transfection, co-transection studies, stable cell line generation, reporter assays, gene expression research, RNA interference, and viral vector production.

• Streamlined Workflows
  Several TransIT® formulations are serum-compatible and do not require media replacement after transfection, simplifying laboratory workflows and reducing hands-on time. 

Solutions for Every Transfection Challenge

The TransIT® family includes specializd reagents developed for different research needs:

• Broad-spectrum DNA transfection for a variety of mammalian cell types.
• Low-toxicity formulations for sensitive and primary cells.
• Enhanced delivery systems for hard-to-transfect cell lines.
• Virus production reagents optimized for AAV and lentiviral vector generation.
• Cell-specific transfection solutions designed to maximize performance in specialized cell models. 

Ideal Applications

TransIT® reagents are widely used in:

• Gene expression studies
• Functional genomics research
• CRISPR and genome engineering workflows
• Stable cell line development
• RNA interference experiments
• Protein production
• AAV and lentivirus generation
• Stem cell and primary cell research 

Trusted Performance Across Research Workflows

For more than two decades, the TransIT platform has been trusted by researchers worldwide for its combination of efficiency, reproducibility, and ease of use. With multiple reagent options available, laboratories can select the optimal transfection solution for their specific cell type, application, and performance requirements while maintaining consistent and reliable results.