AtlasPlex is a complete, ready-to-use kit for multiplex IHC on fixed (FFPE) tissue sections.
With this groundbreaking product, researchers can explore over 13'000 protein targets thanks to the extensive antibody portfolio from Atlas Antibodies, all without the typical challenges of reagent selection or species-dependent workflow optimization. The AtlasPlex kit Includes Biotinylated Antibodies (3 or 5 antibodies for our 3-plex or 5-plex formats), along with relevant reagents to perform 25 or 50 reactions.
Why Choose AtlasPlex?
Challenges researchers face when working with multiplex IHC:
• Limited plex capacity
• Species-dependent reagents
• Difficulty finding suitable, high-quality antibodies
• Weak signals for critical targets
• Complex TSA protocol optimization
• Restricted access to valuable tissue samples
Many are held back by low signal intensity, protocol complexity, and inflexible systems. Moreover, most multiplex kits on the market only add to the problem: they are either premade with fixed gene targets or offer very limited customization, often focused on narrow fields like immuno-oncology.
AtlasPlex is different. It’s the only solution that lets users easily design a kit with access to over 12'000 target genes, enabling truly customizable panels for a wide range of research areas using a simple and intuitive process. AtlasPlex was created to solve the limitations of conventional multiplex IHC. It offers a flexible, ready-to-use solution that expands marker capacity, simplifies workflows, and delivers
strong, reliable signals empowering researchers to move faster and go further in their work.
Key Advantages of AtlasPlex
| Ready-to-use Kit | Available in 3-plex or 5-plex formats, eliminating the need for secondary antibodies. |
| Custom Panel Options | Choose from ~12,000 targets from thousands of antibodies from the Human Protein Atlas, allowing for tailored panels based on specific research needs. |
| Efficient TSA Workflow | Allows for strong signal amplification, no harsh stripping, and better tissue preservation. |
| Rapid Results | From sample to result in a single day. |
| Expert Support | Free panel design support from our knowledgeable team. |
| Affordable Pricing | Get everything in one click. All components included. No need to buy expensive reagents. |
| No need for expensive automation | Works on standard fluorescence microscopes. |
Atlas Plex Workflow: Iterative, Multiplex Detection
AtlasPlex enables iterative multiplexed immunohistochemistry for 3 or 5 protein detection in fixed tissue.
In the workflow, biotinylated primary antibodies (Ab1,...Ab5) are sequentially pre-complexed with StreptaClick®-HRP, allowing for precise, enzyme-mediated tyramide signal amplification.
Each cycle follows the same sequence:
(1) Antibody Complexing: The selected biotinylated antibody is bound to StreptaClick®-HRP.
(2) Tissue Incubation: The Ab-HRP complex is applied to fixed tissue for specific antigen binding.
(3) Tyramide Amplification: HRP catalyzes fluorescent signal deposition at the antigen site.
(4) HRP Quenching: The enzyme is inactivated to prevent cross-reactivity in the next round.
Cycles 1- 4 are repeated up to 5 times using 5 different biotinylated antibodies.
(5) Imaging: The final slide is imaged using standard fluorescence microscopy, producing clear, high resolution multiplexed images for spatial analysis and biomarker discovery
Supplier
Atlas Antibodies
Atlas Antibodies is the original manufacturer of highly validated primary antibodies and control antigens. The company was founded by researchers from the prestigious Human Protein Atlas project who wanted to make the unique antibodies developed in the project available to fellow researchers worldwide.
Atlas Antibodies offers curated panels for specific research areas, including Neuroscience, Cell Cycle & Proliferation, Cancer Biology and more.