CELLBANKER® Cell Freezing Media - Protect your frozen cells and tissues
The CELLBANKER® series offers a comprehensive range of cryopreservation solutions designed for high cell viability, recovery, and ease of use. With ready-to-use formulations that eliminate the need for complex preparation, CELLBANKER® ensures reproducibility and long-term stability across diverse applications, including stem cell and organoid storage, as well as GMP-compliant bioprocessing. Options include serum-free, animal-free, and customized formats, making it the ideal choice for research applications.
Why Use the CELLBANKER® Range?
Cited in over 1'700 scientific publications, the CELLBANKER® range is a trusted solution for the storage of any cell types.
- Long-term stability: proven to maintain cell integrity for >8 years at -80˚C or -196˚C
- High cell viability: maintains >90% viability post thaw
- Validated for a broad range of cell types: including primary cells and stem cells.
- No need for a programmable freezer: no expensive, specialized freezing equipment needed
- Ready-to-use formulation: Simplifies workflows with no need for further preparation
- Matches your needs: Serum, serum-free and chemically defined formulations
- Long shelf life: 3 years from manufacturing date
Discover the CELLBANKER range
Available in several formulations, this series of easy-to-use cell freezing media offers high cell viability in serum, serum-free, GMP and DMSO-free formats.
CELLBANKER® 1 Serum Containing Freezing Media
CELLBANKER® 1 is a ready-to-use serum containing cell cryopreservation medium designed for a broad spectrum of mammalian cells. This unique formulation allows for stable cryopreservation and high viability after freeze-thaw cycles, even for sensitive cells. Contains serum, DMSO, glucose, salts and buffer.
CELLBANKER® 2 Serum-free Freezing Media
CELLBANKER® 2 is a serum-free cell cryopreservation medium optimized for serum-free cultured cells and peptide/protein expressing cells. CELLBANKER® 2 offers a low cost-animal free cryopreservation media for all applications where risks of contamination must be avoided. Allows cell cryopreservation directly at -80°C without requiring a rate controlled freezer. Contains no animal-derived products and is guaranteed sterile.
STEM-CELLBANKER® Stem Cell Freezing Media
Animal-free stem cell freezing media optimized for iPS and ES cells
STEM-CELLBANKER® is a chemically defined freezing media optimized for stem cells and iPS cells storage, as well as fragile primary cells. Furthermore, recent data supports its ability to cryopreserve organoids and tissues to allow the recovery of viable cells.
- Chemically defined and animal product free
- FDA Drug Masterfile registered
- Manufactured in compliance with US, EU and PIC/S GMP guidelines
- Maintains pluripotency post thaw
- Enables long term cell storage at -80˚C or -196˚C
- Direct freeze to -80˚C with no rate controlled freezing
Featured products
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| 11911 | CELLBANKER 1 (20ml) | 20 ml | 35.00 |
| 11910 | CELLBANKER 1 (100ml) | 100 ml | 140.00 |
| 11884 | CELLBANKER 1 (4 x 20 ml) | 4 x 20 ml | 135.00 |
| 11914 | CELLBANKER 2 (100ml | 100 ml | 135.00 |
| 11922 | STEM-CELLBANKER - GMP Grade (20ml) | 20 ml | 60.00 |
| 11924 | STEM-CELLBANKER - GMP Grade (100ml) | 100 ml | 255.00 |
| 11894 | STEM-CELLBANKER - GMP Grade (4 x 20 ml) | 4 x 20 ml | 230.00 |
| 13925 | STEM-CELLBANKER DMSO FREE - GMP Grade (20ml) | 20 ml | 60.00 |
| 13926 | STEM-CELLBANKER DMSO FREE - GMP Grade (100ml) | 100 ml | 255.00 |
| 11894F | STEM-CELLBANKER DMSO FREE - GMP Grade (4 x 20 ml) | 4 x 20 ml | 235.00 |
Cryopreservation procedure for cultured cells
- Examine and make sure the cell culture is free of contamination, in a healthy situation and proper confluency, etc. Perform a cell count to determine the viability of cells.
- Gently pellet the cells by centrifugation (3 - 5 minutes at 1,000~2,000rpm, 4oC). Remove the supernatant by using an aspirator.
- Gently suspend the cells with CELLBANKER® cell freezing media
- Dispense the cell suspension in aliquots to cryopreservation vials that have been labeled with the cell line name, cell concentration, passage date and other essential information.
- Place the vials directly in a -80°C freezer for storage. If necessary, transfer the frozen vials to a liquid nitrogen storage tank after the vials have been frozen for at least 24 hours.
Please note: Optimum protocol may change with the cell types
Cryopreservation of organoids
Organoids are 3D, organ-like structures derived in vitro via self-assembly of pluripotent stem cells or adult tissue stem cells. CELLBANKER media has been validated for cryopreservation and recovery of whole organoids, with high viability rates. Clones with desired genetic characteristics (e.g. CRISPR/Cas9 edits) frozen in CELLBANKER media have also been successfully thawed and grown into organoids.
Further resources
Application examples
- Breakthroughs in Neuromuscular Disease Research through 3D Modelling
- Cell surface levels of Heparan Sulfate preserved using CELLBANKER 2
Selected citations
- PBMCs - Hiroyoshi. D. et al. (2018) Hepatology - Advanced cirrhosis drives enhanced B-cell differentiation resulting in hyperglobulinemia.
- Hepatic stellate cells - Nakamura A et al. (2010) Med Mol Morphol. 43:107–115 - Human primary cultured hepatic stellate cells can be cryopreserved
- Primary human hepatocytes - Saliem M et al. (2012) World J Hepatol. May 27;4(5):176-83. - Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution
- Amniotic epithelial cells - Miki T et al. (2016) Stem Cell Research & Therapy 2016 Jan 12;7:8 - Biological impact of xeno-free chemically defined cryopreservation medium on amniotic epithelial cells.
- iPSCs & embryonic stem cells - Holm et al. (2010) Hum Reprod. 25(5):1271-9. Epub 2010 Mar 5. - An effective serum- and xeno-free chemically defined freezing procedure for human embryonic and induced pluripotent stem cells.