FastGene qFYR Real-Time PCR System
- Fast detection − Probe & dye-based assays
- Multiplex qPCR − 4 channels
- Standard plate format − 96-well block with gradient function
- Highest sensitivity
- Oustanding homogeneity across all wells of a 96-well plate
- Software included − Easy-to-use and intuitive
- Integrated Precision Melt Analysis Tool (for probe-based allelic discrimination)
- Many applications − Gene expression analysis, genotyping, HRM (high resolution melt), pathogen detection, genetic analysis
Real-time fluorescent quantitative PCR system
The FastGene® qFYR is a highest precision instrument for performing quantitative polymerase chain reaction (qPCR) experiments. qPCR is a well-established method for the sensitive detection and quantification of nucleic acids. During a measurement, the target DNA or RNA sequence is amplified, while a cycle-dependent increase of a fluorescent signal is detected in real-time.
| Cat-No. | Item | Size | Price (CHF) |
|---|---|---|---|
| FG-QPTC01 | FastGene qFYR Realtime PCR System | 1 each | price on request |
Specifications
Thermal cycler
| Block capacity | 96 |
| Sample volume | 1–50 µL |
| Heating and cooling method | Peltier (6 temperature control modules) |
| Temperature control technology | Hollow-out module combined with edge temperature compensation technology |
| The maximum heating and cooling rate of the block | 6.0 °C/s |
| The average heating and cooling rate of the sample | 4.0 °C/s |
| Temperature range | 4–100 °C |
| Temperature accuracy | ± 0.2 °C |
| Temperature uniformity | ± 0.2 °C |
| Temperature setting range | 4–100 °C |
| Heated lid | Electronic automatic lid |
| Gradient zones | 12 columns |
| Temperature gradient temperature range | 1–36 °C |
| Linear dynamic range | 10 orders of magnitude: 1-1010 copies |
| Software name | FastGene®qFYR Analysis Studio |
Optical detection
| Excitation light source | 4 single-color high-efficiency LEDs (maintenance-free, working life >100,000 hours) |
| Detector device | Highly sensitive PMT (photo multiplier tube) with Frensel lens |
| Scanning principle | Time-resolved real-time scan |
| Fluorescent channel number | 4 channels (2x FAM) |
| Application to fluorescent | Channel 1: FAM/SYBR Green etc. Channel 2: VIC/JOE/HEX/TET etc. Channel 3: ROX/Texas Red etc. Channel 4: Cy5 etc. Channel 5: FAM/SYBR Green etc. |
| Dye compatibility | FAM/SYBR Green VIC/HEX/TET/JOE ROX/Texas Red, Mustang Purple Cy5/LIZ |
| Detector position | Top of the block |
| Detection sensitivity | 1 copy of the target sequence |
| System sensitivity | Distinguishable 1.33-fold copy number difference in singleplex reactions |
| Detection time | Standard mode (full channel): 8.5 seconds/96-well plate; Fast mode (dual FAM): 4 seconds/96-well plate |
| Exitation/detection range | Excitation range: 455–650 nm; Scope of test: 510–715 nm |
Physical specifications
| Instrument specifications | Dimensions: 35 cm x 52 cm x 37 cm (length x wide x height) Weight: 25 kg |
| Environmental conditions | Temperature: 10–30 °C Humidity: ≤85 % RH no condensation Atmosheric pressure: 85.0 kPa ~ 106.0 kPa Altitude: < 2000 m |
| Power supply specification | Power supply voltage: AC 100–240 V Frequency: 50 Hz Power: 750 W |
| Data communication interface | USB ports |
High sensitivity optical design
The FastGene® qFYR has a unique, patented optical detection system. It combines a high quality PMT (photomultiplier tube) with a Fresnel lens that has a short focal length. The resulting short distance from detector to sample reduces signal loss and cross-talk between samples, and generally improves signal sensitivity.
Superior thermal cycler
The FastGene® qFYR delivers excellent temperature control, temperature precision (± 0.2 °C) and uniformity over the entire 96-well plate. The unique hollowed out thermal block design reduces its overall weight and ensures rapid heating and cooling rates (up to 6 °C per second) for fast qPCR protocols. The system also uses state-of-the-art Peltier components for highest reaction quality and performance stability, providing reliable and consistent qPCR results.
Video Software Explanation
In this video we show you how simple it is to set up a qPCR experiment with the FastGene® qFYR software. We show the software installation, experiment setup and data analysis.
Powerful User Software
The analysis software (FastGene® qFYR Analysis Studio) impresses with its particularly simple and operation. The menu is clearly structured and intuitively arranged. It responds to the needs of the user for different experimental setups, and personalized settings can be easily adjusted. Integrated analysis algorithms allow many steps, such as baseline subtraction or Cq value threshold calculation, to be performed automatically.
Absolute and relative quantification of nucleic acids can also be automated. The software allows you to save predefined analysis settings for auto-exporting run data into a format of choice, including Excel, PDF, txt export format.
- Clearly structured and user-friendly
- Intuitive navigation
- Customizable settings
- Automatic analysis with integrated algorithms
- Modules for multiple applications
- Modern UI design
Nippon Genetics - reagents & tools for Molecular Biology
Midori Green safe DNA stains, DNA/RNA visualization, gel documentation, RNA & DNA purification, cell freezing & storage, filter tips & PCR tubes & plates.