PKmem dyes were developed by the lab of Zhixing Chen at Peking University. They label the plasma membrane in live cells with very high specificity. The unique and unmatched feature of PKmem dyes is their extremely low phototoxicity, due to the presence of the intramolecular triplet quencher cyclooctatetraene (COT) group. PKmem probes allow long term live cell imaging of the plasma membrane without damaging the cells and they do not require any genetic manipulation, transfection or overexpression of fluorescent proteins.
Key features
- Fast staining within 5-15 minutes
- Massively reduced phototoxicity compared with other membrane probes
- Highly specific staining of the plasma membrane
- SIM and STED compatible
- Compatible with PFA fixation and immunostaining
PKmem 555
| Absorbance maximum | 556 nm |
| Emission maximum | 572 nm |
| STED depletion wavelength | 660 nm |
| Works on fixed cells? | Yes |
PKmem 590
| Absorbance maximum | 590 nm |
| Emission maximum | 606 nm |
| STED depletion wavelength | 775 nm |
| Works on fixed cells? | No |
PKmem 650
| Absorbance maximum | 650 nm |
| Emission maximum | 671 nm |
| STED depletion wavelength | 775 nm |
| Works on fixed cells? | Yes |
Ordering information
| Catalog No. | Item |
|---|---|
| SC062 | PKmem 555 |
| SC063 | PKmem 590 |
| SC065 | PKmem 650 |
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Publications
| Filename | Download |
|---|---|
| Ling_et_al_2024_PKmem_probes.pdf |  |