What are eBlocks?
eBlocks Gene Fragments are double-stranded DNA fragments of 300-1500 bp in length. They are uniquely suited for high-throughput screening of multiple constructs, especially for antibody discovery scientists who want to obtain DNA constructs quickly and reliably for most cloning applications.
- FAST time to ship (1–3 business days from order confirmation)
- Budget friendly
- Low screening effort to find your clone
New - eBlocks Gene Fragments up to 1'500 bp
We’ve improved our eBlocks™ Gene Fragments
IDT now offers eBlocks Gene Fragments up to 1'500 bp. Whether it’s for a routine cloning or high-throughput screening experiment, we’re providing you with more flexible options when designing your DNA sequences—without compromising quality or cost.
eBlock specifications
| Specification | Information |
|---|---|
| Format | Delivered in plates |
| Minimum order | 24 fragments |
| Length | 300-1500 bp |
| Yield | 200 ng in 20 ul |
| Concentration | 10 ng/ul |
| Lead time | 1-3 days |
Performance
Consistent high fidelity
All IDT gene fragments demonstrate a consistent, high sequence fidelity across various lengths, and eBlocks Gene Fragments are no exception (Figures 1 and 2).
Figure 1. The effect of error rate on predicted cloning success.
With a median error rate of less than 1:5000, eBlocks Gene Fragments demonstrate a high probability of first-time cloning success. This indicates that IDT Gene Fragments are more likely to give a correct clone the first time than fragments supplied by other vendors at comparable length ranges
Figure 2. Demonstrated cloning efficiency.
Compared to a leading provider, eBlocks Gene Fragments showed a significant improvement in cloning efficiency, leading to a reduction in the time and cost to find a correct clone.
Robust and flexible assembly
Thorough quality control testing of each eBlocks Gene Fragment ensures consistently high cloning rates for generating recombinant colonies. eBlocks Gene Fragments are compatible with many convenient cloning and assembly kits. Table 2 demonstrates the number of colonies needed to be picked to have a >90% chance of obtaining a desired clone.
Table 2. Low screening effort is needed for eBlocks Gene Fragments to find the correct clone.
| Length | Colonies to pick |
|---|---|
| 300–499 | 2 |
| 500–1500 | 3 |
For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.