Still using Digitonin to stabilize your membrane proteins?
Here are 4 reasons why you should switch to GDN
1. No risk of toxic byproducts
While digitonin is a natural product and can contain harmful byproducts such as digitoxin and digoxin, GDN is produced synthetically, without toxins.
2. No batch-to-batch variation
Again, as a synthetic product, GDN has the benefit of not varying between batches as digitonin does.
3. Lower CMC
GDN's critical micelle concentration is 18uM - way lower than digitonin's 0.25-0.5 mM!
4. Better water solubility
With ≥10% water-solubility at RT, GDN beats digitonin even here!
Have we convinced you yet?
At LubioScience, we offer Anatrace’s high-quality GDN101 (≥98% purity by HPLC analysis) in multiple sizes between 500 mg and 100 g.
Literature for GDN
Description of GDN and performance comparison to DDM:
Some recent Cryo-EM studies using GDN instead of Digitonin:
- Koehl, A., et al. (2018) Nature 558(7711): 547–552.
- She, J., et al. (2018) Nature 556(7699), 130–134.
- Hartler, A. M., et al. (2019) Nat Struct Mol Biol. 26(1): 78–83.
- Walter, J. D., et al. (2019) eLife 8: e46986.
- Qian, H., et al. (2019) Nat Commun. 10: 2320.
- Shanmuganathan, V., et al. (2019) eLife 8: e46267.
- Singh, A. K., et al. (2018) Nat Struct Mol Biol. 25(9): 805–813.