CRISPR/Cas9 tutorial from IDT
Optimized methods to use Cas9 nickases in genome editing
Literature
Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E (August 2012). “A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity”. Science. 337(6096): 816–821. Bibcode:2012Sci...337..816J. doi:10.1126/science.1225829. PMC 6286148. PMID 22745249.
Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (February 2013). “Multiplex genome engineering using CRISPR/Cas systems”. Science. 339(6121): 819–823. Bibcode:2013Sci...339..819C. doi:10.1126/science.1231143. PMC 3795411. PMID 23287718
Pennisi E (August 2013). “The CRISPR craze”. News Focus. Science. 341(6148): 833–836. Bibcode:2013Sci...341..833P. doi:10.1126/science.341.6148.833. PMID 23970676.
Zhang F, Wen Y, Guo X (2014). “CRISPR/Cas9 for genome editing: progress, implications and challenges”. Human Molecular Genetics. 23(R1): R40–6. doi:10.1093/hmg/ddu125. PMID 24651067.
Hsu PD, Lander ES, Zhang F (2014). “Development and applications of CRISPR-Cas9 for genome engineering”. Cell. 157(6): 1262–1278.
Lander ES (January 2016). “The Heroes of CRISPR”. Cell. 164(1–2): 18–28. doi:10.1016/j.cell.2015.12.041. PMID 26771483.
Quadros RM, Miura H, Harms DW et al. (2017). “Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins”. Genome Biology. 18: 92.
Jacobi AM, Rettig GR, Turk R, Collingwood MA, Zeiner SA, Quadros RM, Harms DW, Bonthuis PJ, Gregg C, Ohtsuka M, Gurumurthy CB, Behlke MA (2017). “Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes”. Methods. 121–122: 16–28.
Additional resources
- Sander & Young review of CRISPR-Cas9 (Nat. Biotechnol.)
- In-depth explanation of CRISPR-Cas9 systems
- CRISPR-Cas9 for genome editing
- Getting started with CRISPR-Cas9 (video)
- Mapping the genomic landscape of CRISPR–Cas9 cleavage (Nat. Methods)
- Nucleic acid detection with CRISPR-Cas13a/C2c2 (Science)
- Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides (PLoS ONE)
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