FuGENE® offers a comprehensive portfolio of best-in-class transfection reagents for the delivery of DNA, RNA, and CRISPR/Cas9 into a wide variety of biologically relevant cell lines, including both primary and established lines. For over 25 years, FuGENE® has supported scientists with reagents that deliver high transfection efficiency while remaining gentle on cells. Designed for applications ranging from gene expression and knockdown to protein production, virus generation, and therapeutic research, FuGENE® reagents provide reproducible performance with low cytotoxicity across a broad spectrum of cell types.

Trusted by researchers worldwide, FuGENE® products are referenced in thousands of publications and supported by robust protocols for virtually any experimental need. 

Cell culture
Molecular biology
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Product offering

  • DNA Transfection Reagents

    • FuGENE® 4K
      High-efficiency DNA delivery with minimal cytotoxicity; ideal for a wide variety of eukaryotic cell types.

    • FuGENE® 6
      Reliable and flexible reagent with excellent performance and low toxicity for routine transfection workflows.

    • FuGENE® HD
      Optimized for hard-to-transfect and sensitive cells; combines strong expression levels with gentle handling of cells.

  • RNA Transfection Reagent

    • FuGENE® SI
      Designed for the delivery of siRNA, miRNA, and other small RNAs; enables efficient gene knockdown with high viability and minimal off-target effects.

  • Applications

    • Transfection of DNA and RNA into mammalian and insect cells
    • Gene knockdown and RNA interference (RNAi)
    • Protein and antibody production
    • Virus production
    • CRISPR/Cas9 delivery
    • Research and development in molecular and cell biology

Which FuGENE reagent is the right one for me?

FuGENE 4Kbest-in-class reagent for highest transfection efficiencies and difficult to transfect cells
FuGENE HDfor protein and virus production, delivery into primary and stem cells
FuGENE 6affordable, reliable transfection of most common cell lines
FuGENE SIsuperior delivery of siRNAs, miRNAs, short RNA molecules

 

FuGENE 4K

  • Outperforms Lipofectamine® 3000 in most cell types
  • Most effective broad spectrum DNA transfection reagent
  • Maximum transfection efficiency in adherent cells
  • Robust performance in suspension adapted 293 & CHO cells
  • Improved viral titers and protein production yields

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FuGENE HD

  • Transfection of difficult-to-transfect cell lines
  • Superior efficiency with minimal-to-no toxicity
  • Maximizes protein, antibody, and virus production
  • Quick protocol with easy optimization steps

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FuGENE 6

  • Over 1000 cell types successfully transfected
  • High efficiency transfection with minimal toxicity
  • Quick protocol with easy optimization steps
  • Cost-effective for budget-conscious laboratories

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FuGENE SI

  • Maximum efficiency & gentle siRNA transfection reagent
  • Utilize lower amounts of transfection reagent and siRNA 
  • Superior gene silencing with minimal-to-no toxicity
  • Simple, easy-to-use protocol and optimization

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