Seminars: CRISPR enzyme optimization in Basel, Lausanne & Zurich
Lai Chan, IDT's European Application Manager, will be speaking at three seminars in the second week of March, 2020, in Basel, Lausanne and Zurich. His presentation will review applications, including gene disruption and homology-directed repair (HDR) using both Cas9 and Cas12a systems.
After the seminars, food and drinks will be provided.
16:30–17:15/18:00 Zentrum für Lehre & Forschung | 11:15–12:00/13:00 UNIL – Génopode | 12:15–13:00/13:30 Uni Zürich, Campus Irchel |
Seminar summary
We will review how IDT developed the most potent set of genome editing tools on the market by combining the precision of chemically synthesized guide RNAs with high performance Cas9 and Cas12a (Cpf1) proteins.
The Alt-R CRISPR system offers powerful on-target editing and reduced off-target effects compared to traditional approaches. This system is non-toxic and easy to deliver into a wide array of cells by lipofection or electroporation. We will present a novel high-fidelity Cas9 mutant that has been optimized to further reduce off-target gene editing while maintaining on-target potency. In addition, we will discuss a novel Cas12a(Cpf1) mutant with dramatically improved cleavage activity that rivals Cas9 and has improved low temperature cleavage activity making it a universal Cas12a (Cpf1) solution for genome editing in both animal and plant species.