Sino Biological is a producer of high quality proteins, cytokines, growth factors, antibodies, ELISA kits and cDNA ORF clones. The portfolio includes more than 10’000 recombinant proteins and 19’000 antibodies, covering a broad range of life science applications such as immunology, cell and developmental biology, oncology, neuroscience, and viral & stem cell research.
Sino Biological has a strong focus on quality, is ISO 9001:2008 certified and is capable of producing under GMP rules.
Portfolio
- Recombinant proteins
- Cytokines & growth factors
- Antibodies and ELISA kits
- cDNA expression clones and qPCR primers
- Cell lysates
- Cell culture media
- Custom and CRO services
Sino Biological specializes in recombinant protein production, antibody development and customized services. All of Sino Biological's products are independently developed and produced, including recombinant proteins, antibodies and cDNA clones.
Their product quality control indicators meet rigorous requirements for clinical use samples. It takes only a few weeks for them to produce 1 to 30 grams of purified monoclonal antibody from gene sequencing.
5 reasons to choose Sino Biological
Sino Biological has built expertise in recombinant production for over 17 years.
- One-stop antibody & protein CRO service provider.
Optimized expression platforms ensure the highest chances of success.
HEK293/CHO transient expression platform & stable cell line development platform. - Customized antibodies & proteins delivered in as fast as 2 weeks.
- 24 well plates to 25 L bioreactors for your screening to production level protein needs.
- State-of-the-art equipment & minimal endotoxin during processing.
- A world-class team of industrial professionals & academic experts.
- All projects led by PhD-level project managers
State-of-the-art equipment
Recombinant protein production
With 1'000+ new proteins in R&D each year, Sino Biological has extensive experience in producing recombinant proteins such as transmembrane proteins, enzymes, kinases, viral antigens, etc. Sino Biological provides complete services from gene cloning and synthesis to protein characterization. Each project is designed with checkpoints that can provide you with decision-informing data before proceeding to the next step.
Multiple protein expression systems are available including those of insect cells, mammalian cells, and E. coli. No expression system is perfect, and each recombinant protein must be evaluated for which system will work the best. Each protein expression system falls on a continuum of worst to best for characteristics such as speed, cost, glycosylation, and folding.
Premium recombinant proteins
- Five expression systems: > 85% produced in eukaryotic cells for protein structures close to native conformation
- Multiple species
- 80+ automated bioreactors (2L-2000L): Scalable production from milligram to gram quantities
- High sability
- Validated bioactivity
- Low endotoxin levels: < 0.1 EU/μg (LAL-tested)
- Carrier-free formulation: Dissolved in PBS, BSA-free
- High purity: >95% by SDS-PAGE or HPLC (see image below)
Biomolecular interaction analysis with BLI
Sino Biological provides high-quality integrated molecular interaction analysis services, including antibody screening, activity detection, epitope binning, consistency evaluation, and quality control services for clients utilizing BLI (bio-layer interferometry) or SPR (surface plasmon resonance) technology.
- Affinity assay of FC receptor protein and antibody
- Affinity of complement and antibody
- Antibody screening and characterization
- Biomolecular interactions
- Small-molecule affinity determination
- Consistency evaluation
Affinity is a characteristic parameter that can be used to evaluate the interaction between two molecules in a reversible reaction process. It is also an important indicator to understand molecular and identify biological functions as well as drug discovery and screening. Protein interaction/ affinity tests are widely used for early phase drug research and development, drug screening and discovery, preclinical and clinical studies, and quality control in downstream production. They are also widely applied in fields such as basic life sciences research and biopharmaceutical development. In industry, technologies such as SPR (Biacore) and BLI (Octet) are commonly used to detect protein interactions.
SPR platform (Biacore 8K and Biacore T200) is a label-free system based on surface plasmon resonance (SPR) technology that monitors biomolecular interactions in real-time. It is applicable for early phase drug research and development as well as quality control in downstream production.
BLI platform (ForteBio Octet RED384), which utilizes diverse sensors, offers high flexibility and sensitivity, enabling accurate analysis for intermolecular affinity.
Endotoxin-free protein platform
Endotoxin-free recombinant proteins are essential for advancing scientific research and drug development, enabling accurate and reliable results in endotoxin-sensitive applications. Endotoxins (Lipopolysaccharide, LPS) have a significant impact on immunology research, vaccine development, monoclonal antibody production, cell and gene therapy, and therapeutic protein production:
- Endotoxins significantly trigger the innate immune response, strong inflammatory, and antimicrobial responses
- Endotoxins interfere with protein processing and biopharma production by binding to proteins, causing aggregation, and competing for binding sites.
- Exceptionally low endotoxin levels (<0.05 EU/mg), exceeding industry standards (0.5 EU/mg according to USP <85>).
- High Purity: purity > 90% by SDS-PAGE & SEC-HPLC
- Best for pre-clinical animal studies, accurate cell assays and detection assays
- Rigorous quality control ensures lot-to-lot consistency and minimal endotoxin levels
- Ideal for animal immunogenicity studies and injections, significantly reducing endotoxin-induced immune responses
- Ready to use, no reconstitution is needed
Recombinant antibody development & production
Sino Biological is committed to providing scientific research institutions and pharmaceutical companies with one-stop custom antibody production services, from antigen synthesis to antibody generation and manufacturing.
With years of experience and expertise in the development and production of custom antibodies, we will support every custom project in an efficient and cost-effective manner.
General workflow of antibody development services
General workflow of antibody production services
Process development and optimization
Contact us if you want to learn more about Sino Biological.
Highly pure and functional cytokines, growth factors and recombinant proteins from Sino Biological
Sino Biological offers a wide range of highly pure, functional cytokines and other recombinant proteins purified from E. coli and mammalian cells.
- Guaranteed to be biologically active
- Low endotoxin levels
- Animal-free and carrier-free, supplied in PBS
- High purity (> 95%)
- Quality control documentation
- GMP-grade cytokines available
Cytokine Guide for Researchers
The comprehensive guide to choosing high-quality cytokines based on applications.
Selection of available cytokines
| Cat-No. | Cytokine | Size | Source |
|---|---|---|---|
| GMP-10395-HNAE | Human IL-6 | 20 ug / 100 ug | E. coli |
| GMP-10015-HNAH | Human GF-CSF | 20 ug | HEK293 |
| GMP-10602-HNAE | Human TNF-alpha | 50 ug / 100 ug | E. coli |
| 11066-HNAH | Human VEGF alpha | 5 ug / 20 ug / 100 ug | HEK293 |
| 10134-H08Y | Human CCL2, His-tag | 20 ug / 50 ug | Yeast |
| 50482-MNCH | Mouse EGF | 100 ug / 500 ug | HEK293 |
| 14890-HNAH | Human LIF | 20 ug / 50 ug | HEK293 |
| 10804-HNAC | Human TGF beta | 5 ug / 20 ug / 100 ug | CHO |
| 67002-UNAE | Feline IL-1 | 5 ug / 10 ug / 20 ug | E. coli |
| 90174-C08H | Cynomolgus CD14, His tag | 50 ug / 100 ug | HEK293 |
Growth factors, cytokines and recombinant proteins
Sino Biological offers a wide range of cytokines, growth factors and other recombinant proteins manufactured to the highest quality standards:
- Produced in mammalian cells
- Ultrapure (> 90% by SDS-PAGE)
- Low endotoxin levels
- Carrier free: dissolved in PBS, without BSA
- 600+ GMP-level cytokines
- Bulk quantities available
Custom production and CRO services
Sino Biological offers a range of custom and CRO services for academic and industry researchers:
- Custom protein expression and purification
- Custom antibody production (mouse monoclonals and rabbit monoclonals)
- Antibody expression (from hybridoma and cell lines)
- Antibody engineering and humanization
- Gene cloning and expression vector construction
SARS-CoV-2 products
Sino Biological is currently offering various SARS-CoV-2 viral proteins and antibodies to aid research. They are listed on our SARS-CoV-2 product page along with other reagents.
- Antibodies against spike protein, nucleoprotein, other SARS-CoV-2 proteins
- Full-length spike protein and all identified variants
- Spike protein fragments - RBD, S1, S2
- ACE2
- Antibody pairs, antibody ELISA sets
- ELISA panels and kits
- ORF expression clones encoding viral and host proteins
Downloads
| Filename | Download |
|---|---|
| Antibody catalog |  |
| Cytokine catalog | |
| Protein catalog | |
| Virus research tool brochure | |
