Molecular Dimensions presents their newest protein stability screen: the Durham Osmolyte Screen
Osmolytes are uncharged, highly soluble organic molecules whose role in cells is to balance high salt external environments. This means they are often present in the environment of native proteins and, in combination with their neutral chemical characteristics, can help stabilize proteins without affecting their function. Researchers in the field of structural and functional biology can thus benefit from the stabilizing effects of these cell components while minimizing adverse impacts on the interpretation of their data. The Durham Osmolyte Screen includes over 50 different osmolytes such as sugars, amino acids, polyols and NDSBs in order to increase the likelihood of finding stabilizing conditions for the protein of interest and to lead to better results.
The Durham Osmolyte Screen is the third ‘Durham’ protein stability screen developed by Professor Ehmke Pohl and his group at Durham University. All three screens (MD1-101 Durham pH Screen, MD1-102 Durham Salt Screen, MD1-120 Durham Osmolyte Screen) are complementary and can be used together to determine the optimum conditions for your protein of interest. In addition, data from any of the Durham Screens can easily be analysed with NAMI, a free-to-download GUI-based software.