Blog

A novel approach to DNA storage architecture

A team of researchers at ETH Zurich has developed a DNA-based storage architecture to produce materials with immutable memory, which could be used to store electronic health records or facilitate the development of self-replicating machines.

An Introduction to multiplex immunohistochemistry

With the advancement of immunotherapeutics, the urge to understand the tumor microenvironment has never been more pressing. Immunohistochemistry is a powerful tool used to examine protein expression, distribution, and activation in situ.

Reduce WB background through proper sample preparation

Many factors can contribute to high background in WB, such as improperly diluted antibodies, insufficient blocking, interference from blocking reagents, insufficient washing and poor quality of transfer membranes.

Guidelines for achieving high rates of HDR with CRISPR Cas12

A novel Cas12a enzyme variant, Alt-R A.s. Cas12a Ultra, greatly increases rates of homology directed repair (HDR) and expands the number of target sites available for editing. In addition, Cas12a Ultra is active at lower temperatures, enabling genome editing of organisms such as fish and plants. The HDR guidelines listed here for optimizing ribonucleoprotein concentration, HDR donor design, and other experimental parameters will show you how to use Cas12a Ultra to increase your HDR rates.

Can You Really Reverse Your Epigenetic Age?

Aging is a normal, and inevitable, part of life. No matter how healthy you are, the process of aging marches on and cannot be stopped or reversed. Well, that’s what we used to think.

Genotyping: Terms to know

Genotyping determines differences in a specific DNA sequence across a population. These sequence variations can be used as markers in linkage and association studies to determine genes relevant to specific traits. Review the vocabulary commonly encountered in genotyping experiments.

NGS target capture recommendations for FFPE samples

Learn how it is possible to create high quality target capture libraries from formalin-fixed, paraffin-embedded samples. Dr Kristina Giorda presents an FFPE sample workflow with a concise explanation of DNA quality analysis and how quality assessment can be used to guide the amount of DNA input for NGS library preparation.

4 tips for oligonucleotide quantification using NanoDrop

Learn how to accurately determine the concentration of your oligonucleotides using Thermo Scientific NanoDrop instruments by following these recommendations from the scientists of IDT.

Ensure reliable qPCR results - advice on assay validation

It's not you, it's your qPCR assay design!

To ensure quantitative, accurate, interpretable results from qPCR, there are several important factors you must consider. Here are a few recommendations for obtaining reliable data, and the link to a free application guide providing best practices and rationale.