Selenozyme is the world's only maker of fully recombinant, active seleoproteins. They are a spin-off company from the research group of Professor Elias Arnér at Karolinska Institutet in Stockhom, Sweden. Their cutting-edge technology is the result from over 30 years of experience and allows for the seamless insertion of selenocysteine into virtually any recombinant protein of interest.
Selenozyme’s active selenoproteins are produced in a bacterial recombinant system. As a result, they can offer you a cost-effective competitive alternatives to selenoproteins purified from native sources.
Product offering
Applications
- Production of fully active, recombinant selenoproteins
- Tagging of recombinant proteins with a Sel-tag (Gly-Csy-Sec-Gly)
Available selenoproteins
- Recombinant human and rat TrxR1
- Recombinant human and rat TrxR2
- Recombinant human GPX1
- Recombinant human GPX2
- Recombinant human GPX4
- Recombinant human GPX4 +
- Recombinant human DIO2
- Recombinant human DIO3
- Recombinant Shistosoma Mansoni TGR
- Recombinant Sel-tagged synthetic selenoproteins
References
Overexpression of Recombinant Selenoproteins in E. coli
Cheng Q and Arnér ESJ. Methods Mol Biol 2018.
Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Escherichia coli Host Strain Bypasses Species Barriers in Recombinant Selenoprotein
Cheng Q and Arnér ES. J Biol Chem 2017.
Tagging recombinant proteins with a Sel-tag for purification, labeling with electrophilic compounds or radiolabeling with 11C
Cheng Q, et al. Nat Protoc 2006.
Exploiting the 21st amino acid – purifying and labeling proteins by selenolate targeting
Johansson L, et al. Nat Methods 2004.
