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DNA and RNA Oligos

Failed clonings? PCR not working?

It may be your oligos.

Did you know that some oligonucleotide manufacturers deliver oligos containing less than 50% full-lenght product?
This means more than half of the oligos in your tube are truncated and missing one or more nucleotides from the original sequence. These oligos create artifacts, such as decreased cloning efficiencies, failed PCR reactions or mistakes in your final construct.

Don't believe it? Take a look at the data below.

We tested 32 oligos of different lengths synthesized either by IDT or by Competitor M. The resulting oligos were analyzed by capillary electrophoresis to determine the percentage of full-length product in each tube. The green peak denotes the full-length oligo, the red peaks indicate truncated byproducts. IDT: 92% full-length product. Competitor M: 52% full-length product. This oligo would have been rejected by IDT's quality control.

Here's more data from the comparison:

Oligo length (nt)% Full-length (IDT)% Full-length (Competitor M)
3088.00%63.62%
3186.40%38.94%
3287.70%55.08%
3380.80%64.66%
3488.60%57.96%
3672.10%49.06%
3792.20%52.66%
3886.10%47.38%
4082.70%47.2%
4284.90%58.54%

On average, IDT's tubes contained 81% full-length oligos, whereas Competitor M's only contained 54% full-length oligos. More than half of Competitor M's oligos would have failed IDT's quality control and would have been remade.

Go for quality - use oligos from IDT in your research

  • Proprietary synthesis platform means highest percentage of full-length oligos in the industry
  • Save time & money: no need for cartridge or HPLC purification
  • Every oligo is fully qc'd by ESI mass spectrometry
  • 500+ modifications available
  • Free 2 day delivery via DHL

Special offer: experience IDT's quality for yourself

Don't take our word for it - test DNA oligos from IDT for yourself right now. We offer up to 10 DNA oligos (25 nmole scale, desalted, no modifications) for free.

Quote "TryIDTOligos". Valid until December 31, 2018, for new IDT customers.

Contact us now

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