Do you need reagents to advance your research that are not available? Or do you lack the in-house expertise or ressources?

This is why LubioScience developed its range of Custom Services. We provide fast and personal consultation and offer full project support to make your custom production experience as smooth as possible. In collaboration with a number of trusted partners, we present you different solutions from which you can choose the most suitable option.

Our custom services offering includes

Bulk Oligo Production

Oligos manufactured to your specifications (length, scale, purity, formulation, dispensing, etc.) to accommodate all your diverse research needs. Obtain high purity DNA and RNA oligos in bulk quantities with competitive pricing. Generate consistently reliable data from the highest fidelity oligos available. All services come with QC documentation and certificate of analysis.

Contact us  to discuss the best solutions for your projects from pilot to large-scale production.

Antibody & Protein Sequencing

Monoclonal antibody sequencing from small protein samples, no need for hybridoma or DNA information. Full sequence in record time.

The REmAb® workflow delivers a full coverage antibody sequence with unparalleled accuracy, 100% of the time (see sample requirements below). Starting with only a small sample of protein, we conduct proprietary chemistry, LC-MS/MS proteomics and machine-learning driven sequencing.

Requirements:

  • any species
  • any antibody isotype
  • 100 ug protein, > 80% purity

Deliverables:

  • The entire mAb sequence averaging 30X coverage per amino acid
  • Independent review – sequences derived by proprietary bioinformatics and expert reviews
  • Isobaric checks – all isobaric combinations are determined by WILD®

Turn around times:

  • rush: 1 week
  • 1 sample: 2 weeks
  • 2-10 sampes: 3 weeks
  • 11+ samples: 4 weeks

REmAb list price:

13'000 CHF

Contact us for more information

Cell line development

Stable cell line shows a great capacity in many fields, especially in immunotherapy, drug screening and gene function study. Our cell line development service includes cell line construction, process development, cell banking and analysis. 

Stable cell-line development service

StepContentTimelineDeliverables
Gene synthesis
  • Gene design including codon optimization
  • Gene synthesis
  • Subcloning in expression vector

3 - 4 weeks

  • Gene optimized for the required expression system
  • Gene cloned in appropriate vectors
  • Sequencing report if required
Stable pool generation
  • Stable transfection
  • Pool selection and amplification
9 - 10 weeks
  • 50mL of the supernatant from stable pool
  • Stable pool vials
  • Detailed report
Single cell clones screening and subcloning
  • Screening for optimal single cell cloning with limited dilution
  • Subcloning of the top producing clones
  • Characterization of clones
2 - 4 months
  • 1-3 single cell clones (2 cryovials/clone)
  • Cell banking
  • Mycoplasma testing
  • Detailed report & CoA
Process optimization (optional)
  • Test of several media/feed conditions
4 - 5 weeks
  • Detailed report
  • Optimized protocol transfer

CRISPR/Cas9 ko cell-line development service

StepContent & deliverables
Host cell characterization
  • Clonality test
  • Transfection efficiency optimization
  • Test the minimal absolute lethal dose of antibiotic concentration

gRNA design and synthesis

  • gRNA design
  • KO vector construction

Transfection

  • Transfection of virus-based delivery
  • Cell pool examination

Single cell screening

  • FACS sorting or antibiotic selection
  • High-throughput screening
  • Expansion of engineered cells

Validation

  • Validation by western blog, ELISA, qRT-PCR, reporter assays, sequencing etc.

Contact us  for a fast & personal consultation.

Gene Synthesis

Complete synthetic genes with 100% sequence verification are provided in a plasmid cloning vector and ready to use in a variety of applications.

  • Avoid spending valuable time and resources on gene construction
  • Use constructs that are manufactured from the highest quality oligos
  • Secure discounts for high volume orders

Contact us  for a fast & personal consultation.

Custom Assay Development

We will work with you to develop, qualify, and manufacture a robust kit that meets your specific process monitoring, release testing, and regulatory needs. We provide comprehensive qualification of your custom assay to meet global regulatory requirements, as well as state-of-the-art orthogonal methods, including our advanced antibody affinity extraction method and mass spectrometry methods.

Contact us  for a fast & personal consultation.

Are you looking for a custom service that is not listed above?

No problem, we are happy to find the right solution for you.

Contact us for a fast & personal consultation