SPY FastAct probes: ultrafast dynamic actin labeling

SPY FastAct™ probes are new and unique fluorescent live cell actin probes. They label very dynamic actin filaments as well as the more static ones. SPY650-FastAct™, SPY555-FastAct™ and SPY700-FastAct™ are bright, fluorogenic, non toxic, cell permeable and highly specific live cell probes for fluorescence imaging of actin. They don't require any transfection and work just by adding the probe to the cells and performing imaging a couple of hours later.

SPY700-FastAct™ is a new and unique fluorescent live cell actin probe. It labels very dynamic actin filaments as well as the more static ones. SPY700-FastAct™ is a bright, fluorogenic, non toxic, cell permeable and highly specific live cell probe for fluorescence imaging of actin. It does not require any transfection and works just by adding the probe to the cells and perform imaging a couple of hours later.

SPY700-FastAct™ is a bright, far red, fluorogenic & non toxic F-actin stain based on our SPY™ dyes series. Its optimized structure allows to label F-actin in live cells with high specificity and low background. The unique and unmatched feature of SPY700-FastAct™ is its ability to label very dynamic actin, like LifeAct™** but the probe does not require any genetic manipulation, transfection or overexpression of fluorescent proteins. SPY700-FastAct™ is based on our bright, photostable & far red SPY700 fluorophore which is far superior than fluorescent proteins.

Image and movie by Maxime Cammeraat, from Margadant lab, Amsterdam, NL. 2 populations of primary human endothelial cells were separately labeled with SPY595-DNA or  SPY505-DNA. After the DNA labeling, the 2 populations were trypsinized, mixed together,  stained with with SPY700-FastAct and imaged.