Isolating DNA from challenging plant and fungi samples
The isolation and purification of DNA from plant tissues is a common practice in research and agriculture industries. Purified DNA is used with a variety of downstream applications such as PCR and sequencing, with the goal of DNA fingerprinting, detecting plant pathogens, genotyping, or detecting genetically modified organisms. The preparation of high quality nucleic acids is essential for successful downstream research and diagnostics. However, environmental samples such as fibrous plants and fungi can prove challenging. Natural samples often contain high levels of PCR inhibitory substances such as soil humic acids and phenolic compounds.
In order to guarantee the isolation of DNA of high quality and integrity, Norgen Biotek offers optimized kits for plant and fungal samples. Their Plant/Fungi DNA Isolation Kit has already been proven to isolate high quality DNA from several plant sample types, such as roots, leaves, stem, fruit skin, and flour from various plant species.
Plant/Fungi DNA Isolation Kit
- Rapid and simple procedure
- Excellent quality and yield of DNA
- Process a broad spectrum of plant species and filamentous fungi (fresh & frozen)
- Isolate total DNA including pathogen DNA without phenol
- For downstream applications incl. PCR, qPCR, SNP, Southern blotting & sequencing
- Available in spin column format and 96-well format for high throughput applications
Plant/Fungi DNA Isolation Kit information sheet Protocol (50 preps)
This kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The protocol can be completed in 45 minutes. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
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|---|---|---|---|
| 58200 | Plant DNA Isolation Kit (Magnetic Bead System) | 50 preparations | 201.00 |
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