Start CRISPRing the easy way with our CRISPR Starter Kit
The CRISPR-Cas9 Starter Kit from IDT supplies all reagents necessary for a complete genome editing experiment.
Use of the CRISPR (clustered regularly interspaced short palindromic repeats) and associated Cas9 enzyme for genome editing has been a major technological breakthrough. The Alt-R® CRISPR-Cas9 System is an optimized genome editing solution that outperforms other CRISPR approaches for producing on-target, double-stranded DNA breaks.
- Controlled delivery, high targeting efficiency, reduced off-target effects
- Chemically modified targeting RNAs for improved stability
- Industry-leading HiFi Cas9 enzymes with unsurpassed specificity
- Precise HDR knock-ins with Alt-R HDR Donor Oligos or
- Alt-R HDR Donor Blocks and Alt-R HDR Enhancer
- Protect your cells from toxicity or innate immune response activation
| Kit Item | Order # | Amount | Description | Price (CHF) |
|---|---|---|---|---|
2 crRNAs | Custom | 2 nmole each | Two custom crRNAs targeting two different loci adjacent to a PAM site in your gene of interest | 220.50 |
tracrRNA | 1072532 | 5 nmole | The tracrRNA assembles together with a custom cRNA and the Cas9 protein into the RNP complex | 120.00 |
Cas9 nuclease | 1081058 1081060 | 100 μg Standard or HiFi Cas9 enzyme, part of the RNP complex | from 250.00 | |
Electroporation | 1075915 | 2 nmole | Enhances efficiency when introducing the RNA complex into target cells via electroporation | 58.00 |
CRISPR-Cas9 | 1072554 1072555 | 1 kit | 1 kit Separate kits for human, mouse, or rat. Includes tracrRNA, HPRT positive control crRNA, negative control crRNA#1, HPRT Primer Mix and Nuclease-Free Duplex Buffer | 190.00 |
Genome editing | 1075931 | 1 kit (25 rxns) | For detection of on-target or known off-target CRISPR events in cultured cells. The T7 endonuclease I (T7EI) mismatch cleavage assay detects on-target genome editing and estimates genome editing efficiency in CRISPR treated cells | 120.00 |
IDTE buffer, | 11-01-02-02 | 10 x 2 ml | 1x TE buffer pH 7.5, nuclease-free, sterile, for resuspension of RNP complexes | 23.00 |
| HDR Donor Oligos | custom | 2 nmole | Single-stranded oligos of up to 200 bases in tubes or plates | 195.00 |
| HDR Donor Blocks | custom | 3 ug | Chemically modified, double-stranded DNA templates, ideal for longer than 120 bp insertions (200 - 3'000 bp) | from 380.00 |
| HDR Enhancer V2 | 10007910 | 30 ug | Small molecule compound to increase the HDR-rate | 125.00 |
For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.