The INDIGlo Luciferase Detection Reagent (LDR) is formulated to deliver a steady, long-lived luminescence signal from mammalian reporter cells expressing firefly luciferase. For convenience, the reagent is provided ‘ready to use’, without the requirement to first re-constitute powder-form components.
How is INDIGlo used?
INDIGlo LDR works equally well in quantifying expressed luciferase activity from 96- or 384-well format reporter assays. The resulting light emission is stable over a 2 hr period (Figure 1), thereby making INDIGlo LDR an ideal, economical detection reagent for both low- and high-throughput batch processing of assay plates.
INDIGlo LDR is formulated as a 2x-concentrated reagent, thereby allowing greater versatility in its use. The 2x-concentrated reagent will be used when using 384-well or 1536-well assay plates that are processed using a homogenous assay strategy.
Alternatively, when processing 96-well assay plates either a homogenous or non-homogenous assay strategy may be used. For convenience, this kit provides an optimized Dilution Buffer for use in diluting INDIGlo LDR when performing non-homogenous assays.
Components
INDIGlo Luciferase Detection Reagent kits provide two reagents in equal volumes:
- INDIGlo LDR, formulated as a 2x-concentrated reagent
- Dilution Buffer, for optional use if performing non-homogenous assays
Performance
INDIGlo LDR performance from both Homogenous and Non-Homogenous assays. Dose-response analyses of Human AhR (INDIGO #IB06001, 96-well assay) was performed using the reference agonist MeBIO. AhR reporter cells were plated across two white assay plates, MeBIO treatment media were added, and the assay plates incubated for 24 hr. Plates were then processed with luciferase detection reagents in either a.) a homogenous or b.) a non-homogenous assay format. Resulting assay metrics are also shown from using another extended-glo detection reagent (Vendor X).
INDIGlo LDR potency is stable following repeated freeze and thaw cycles. INDIGlo LDR was frozen at -20°C and thawed either 1, 2 or 3 times, then used to quantify luciferase activity from HEK-293:luc cells cultured in a white 96-well assay plate. No loss of reagent potency is observed between these conditions.
Manuals
| Filename | Download |
|---|---|
| TM_INDIGlo-Luciferase-Detection-Reagent-v1.2.pdf |  |