High quality recombinant Proteinase K from Cusabio
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
Proteinase K remains active:
- Over a wide pH range (optimal activity between 6.5 and 9.5)
- Under denaturing conditions (e.g., in the presence of SDS or urea)
- In the presence of metal chelating agents (e.g., EDTA)
- At comparatively high temperatures (optimum digestion temperature is 65°C)
Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays.
Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
Product Details
| Purity | Greater than 95% as determined by SDS-PAGE |
|---|---|
| Activity | ＞30U/mg |
| Species | Tritirachium album (Engyodontium album) |
| Mol. Weight | 28.9 kDa |
| Form | Lyophilized powder |
| DNases/RNases | None detected |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Storage Conditions | Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Datasheet | Download Technical Datasheet and sample COA |
Product listing
| Cat-No. | Item | Size | Price (CHF) |
|---|
Are you interested in bulk sizes? Contact us!