CRISPR Seminar Basel
Alt-R CRISPR-Cas9 - An optimized genome editing solution - Unleashing the Potential of Genetic Engineering
CRISPR technology has been a major technological breakthrough, making genome editing easier, faster, more efficient, and more robust than previous methods.
In this seminar you will learn:
- Guidelines when designing a CRISPR experiment.
- Advantages and disadvantages of Cas9 vs Cas12 for gene editing, and their applications.
- gRNA design and formats selection recommendation
- How to optimize cell-editing with high on-target and reduced off-target activity using Alt-R HiFi CRISPR-Cas9 nuclease.
- Considerations to improve HDR (Homology Directed Repair) efficiency for knock-in experiment.
- How chemical modifications for dsDNA donor templates improve the HDR efficiency and reduce blunt insertion events.
- How and where to introduce silent mutations to improve HDR rates
- How Alt-R HDR Enhancer V2 further improves HDR rate.
Speaker
Abdelilah Mekhloufi, Field Application Scientist, Integrated DNA Technologies
Hosts
Dr. Fabien Cubizolles, University of Basel
Hendrik Mallin, Head of Sales & Key Accounts, LubioScience GmbH
When: May 11, 2023, 11.00 CEST
11:00 – 11:45 Seminar
11:45 – 12:30 Free lunch and discussion
Where: Basel (free)
Biozentrum - University of Basel, Room U1.197,
Spitalstrasse 41,4056 Basel
Following the seminar, free lunch and drinks will be provided. We therefore kindly ask you to register.
The number of participants is limited. Hurry now to secure your place!