IDT - Integrated DNA Technologies
Integrated DNA Technologies (IDT) is your advocate for the genomics age. For more than 30 years, IDT's innovative tools and solutions for genomics applications have been driving advances that inspire scientists to dream big and achieve their next breakthroughs.
IDT develops, manufactures, and markets nucleic acid products that support the life sciences industry in the areas of academic and commercial research, agriculture, medical diagnostics, and pharmaceutical development.
IDT is recognized widely as the industry leader in the manufacture of custom oligonucleotides for molecular biology applications. They have developed proprietary technologies for genomics applications, such as next generation sequencing, CRISPR genome editing, qPCR, and RNA interference.
They maintain their leadership position through a commitment to the highest standards of quality, service, and technical expertise, which allows them to adapt to the evolving needs of genomic science. They are continually improving IDT synthesis platforms, processing technology, automation, as well as their rapid prototyping and custom part production.
Integrated DNA Technologies is run by scientists dedicated to helping other scientists answer research questions through collaborative interactions. They serve more than 100'000 life sciences researchers and produce over 65'000 nucleic acids daily.
Browse below for product information and to order.
Custom DNA oligos
Single-stranded and duplexed DNA sequences, manufactured to your specifications and quality controlled by mass spectrometry. Import multiple sequences from an Excel or text file and select from hundreds of modifications. For high-throughput oligo needs, order in plates and receive discounted base pricing.
Custom RNA oligos
Single-stranded and duplexed RNA sequences, manufactured to your specifications. Import multiple sequences from an Excel or text file and select from hundreds of modifications. Choose from a variety of custom services to meet your research needs.
High purity, bulk DNA or RNA oligos with competitive pricing and fast turnaround time, with final yields from 25 mg to 10 g.
The range of modifications IDT offers is unmatched within the industry. Browse their catalog for detailed information on the available modifications, or contact us if you cannot find what you need.
Use of the CRISPR (clustered regularly interspaced short palindromic repeats) and associated Cas9 enzyme for genome editing has been a major technological breakthrough, making genome modification in cells or organisms faster, more efficient, and more robust than previous genome editing methods. The Alt-R® CRISPR-Cas9 System is an optimized genome editing solution that outperforms other CRISPR approaches for producing on-target, double-stranded DNA breaks.
IDT has also developed an alternative Alt-R CRISPR-Cas12a (Cpf1) system to open up CRISPR editing to additional areas in genomes.
The Alt-R CRISPR systems were developed through comprehensive research of each component of the CRISPR-driven, double-stranded break generation that is critical for gene disruption and DNA insertion by homologous recombination.
Alt-R CRISPR-Cas9 system
Efficient CRISPR reagents based on the commonly used Streptococcus pyogenes Cas9 system for lipofection or electroporation experiments. Protospacer adjacent motif (PAM) = NGG.
Recombinant, high-purity Cas9 and Cas12a (Cpf1) endonucleases for genome editing experiments.
Genome editing detection
T7 endonuclease I (T7EI) mismatch cleavage assay for detection of on-target editing, known off-target events, and estimation of genome editing efficiency in cultured cells.
Single- and double-stranded DNA fragments and cloned genes for synthetic biology and other molecular biology applications
IDT's high-fidelity synthetic genes and gene fragments are the result of a long-standing commitment to innovative chemistry, optimized methods, and a robust manufacturing infrastructure. These products are built using their Ultramer® DNA Oligos, which set the standard for quality within the industry, and every gene or gene fragment you receive shares or exceeds that consistently high quality.
gBlocks® - double-stranded DNA fragments
gBlocks® gene fragments for easy gene assembly, genome editing, qPCR standards, and much more. 125 bp to 3000 bp in length, shipped in only a few business days.
Megamers - single-stranded DNA fragments
Megamer single-stranded DNA fragments are sequence-verified, single-stranded DNA strands of length 201–2000 bases. They can be used in applications such as homology-directed repair of CRISPR-mediated genome editing, in vitro transcription, and more.
Ultramer DNA Oligos
Long (up to 200 bp), high-quality oligos for demanding applications such as cloning, ddRNAi, homology-directed repair, and gene construction.
Next generation sequencing (NGS) technologies are rapidly becoming an integral part of genetic research and discovery. The ability to generate large amounts of sequence data in a relatively short amount of time enables a wide range of genetic analysis applications and accelerate advances in research, clinical and applied markets.
Critical to the success of obtaining high quality results in NGS experiments is the availability of high quality tools to effectively prepare fragment libraries representing the original nucleic acid samples. From platform adapters to their portfolio of xGen® products for targeted sequencing, IDT’s mission is to deliver innovative, high performing NGS products to enable you to increase your discovery power.
Adapters for next generation sequencing platforms
Comprehensive offering of custom and catalog adapters for any NGS platform from the leader in nucleic acid manufacturing. Choose from a variety of barcoding and error correcting strategies, including designs with unique dual index (UDI) barcodes and unique molecular indicators (UMIs).
xGen® products for target capture
A suite of high performing enrichment probes, blockers and workflow reagents for targeted sequencing. The xGen® product portfolio enables unparalleled target coverage and uniformity, even across difficult genomic regions containing high GC content.
Polymerase chain reaction (PCR) is a core laboratory method. An enhancement of this method, qPCR (quantitative PCR) measures the amplification of DNA in real time. PCR and qPCR have contributed to significant advances in gene expression, genotyping, cloning, and other applications.
Over several decades, IDT has refined a proprietary platform that improved nucleotide coupling efficiency during oligo synthesis. Subsequent purification steps ensure a high quality primer or probe for consistent performance. All oligos are QC tested in-house by ESI mass spectroscopy to ensure an accurate, full length, final product.
Choose from probe- or intercalating dye–based qPCR options across a flexible, user-defined product offering. PrimeTime® products for gene expression are created to work well together, but can also be ordered and used separately. This modular system includes predesigned or custom assays, custom probes, master mix, and design tools.
Use the rhAmp™ SNP Genotyping System as a fully integrated, high performing solution for out-of-the-box convenience. For user-defined methods, order custom probes with modifications that increase Tm to enable designs within difficult sequences and with selective target identification.
Select from a broad catalog of custom, fluorescent probe options. IDT offers several probe types, including 5′ nuclease hydrolysis probes [with or without locked nucleic acid (LNA) bases], double-quenched hydrolysis probes (with a second ZEN™ or TAO™ quencher), or molecular beacon probes. Probes may be ordered with numerous dye-quencher combinations and formatting options. GMP manufactured probes are also available.
Rely on IDT to provide quality primers needed for consistent results. Their primers can be used as part of a larger product solution or ordered as standalone core reagents. Design and ordering is easy using IDT's free, online tools.
Master mixes, enzymes, and reagents
Select easy-to-use pre-formulated mixes to complete your gene expression or genotyping experiment. Core reagents (RNase H enzyme, buffers, and solutions) are also available for more individualized needs.
As the creator of the ground-breaking gBlocks® Gene Fragments, Integrated DNA Technologies shares over 16 years of synthetic biology experience with you through their innovative product development, real-time web-based order tracking, and experienced customer support staff.