ChromoTek - Home of the alpaca antibodies

NEW Nano-Secondaries: alpaca secondary antibodies with Alexa Fluor conjugation

About ChromoTek

ChromoTek offers innovative research reagents based on camelid antibodies, which are devoid of light chains and have a single antigen recognizing domain. In the absence of light chains, the fragment-antigen-binding (fab) part of these antibodies is reduced to a single domain, the so called VHH (variable domain of heavy chain antibodies) domain or nanobody. This single domain contains a complete antigen binding site and is the smallest functional antigen binding fragment (around 15 kDa, only one tenth the size of a conventional antibody).

The alpaca antibody advantage

ChromoTek’s Nano-Traps are perfect tools for the rapid immuno- precipitation of proteins and their interaction partners with high specificity and efficiency. Their Nano-Boosters enhance the signal intensity of your targets in fluorescent microscopy. And their Chromobodies® allow you to analyze endogenous proteins in living cells in real time. In addition , their new fluorescent 2-hybrid (F2H®) assay enables you to monitor reversible protein-protein interactions in a cellular system. Last but not least, their Spot-System is the first peptide-tag specific nanobody for universal capture and detec-tion applications. click here to read the story

Product offering

Nano-Traps

Nano-Traps are ideal for fast, reliable and efficient one-step immunoprecipitations. They pull down your proteins of interest (POI) and their interacting factors from cell extracts or organelles. Nano-Traps consist of a VHH, also termed nanobody, coupled to an immobilizing matrix. You can use Nano-Traps for a multitude of biochemical analyses such as:

  • Immunoprecipitation (IP) / Co-IP
  • Mass spectrometry
  • Enzyme activity measurements
  • ChIP / RIP analysis

Nano-Boosters and Nano-Labels

ChromoTek's Nano-Boosters/Labels are very small, highly specific binding proteins. They are derived from single-domain alpaca antibody fragments termed VHHs or nanobodies which are covalently coupled to bright and stable fluorescent dyes. As these nanobodies are much smaller compared to conventional primary plus secondary antibodies (IgG complex), they are particularly suitable for effective labelling with minimal fluorophore displacement for super-resolution microscopy. 

While Nano-Boosters, e.g. GFP- and RFP-Booster, enhance the signal of fluorescent proteins, Nano-Labels, e.g. Vimentin-, Histone- and Spot-Label, fluorescently label proteins.

Advantages

  • Higher image resolution
  • Excellent performance in IF and super-resolution microscopy
  • Stabilization, enhancement and reactivation of fluorescent signals
  • Less than 2nm epitope-label displacement minimizes linkage error
  • Monovalent, recombinant single domain antibody fragment from camelid

Nano-Secondaries

Nano-Secondaries are secondary alpaca antibodies for higher resolution and better imaging, which are conjugated to Alexa Fluor® dyes. They can be used for Immunofluorescence, Super-resolution microscopy (SRM) and Western blot. As Nano-Secondaries are recombinantly produced, they are fully sequenced by design, which in combination with high QC standards ensures reliable and stable products virtually without batch-to-batch variation.

Benefits

  • Higher resolution in immunostaining
  • Thoroughly validated for precise and reproducible staining
  • Cross reactivity against multiple species’ antibodies and sera has been carefully determined
  • Fast one-step immunostaining
  • Economical: only small amounts needed

The first product in ChromoTek's Nano-Secondaries line is the Alpaca anti-rabbit IgG, conjugated to Alexa Fluor® 488, 568 or 647. This nanobody is a mixture of two monoclonal antibodies that recognize Fab and Fc fragments of rabbit IgG.

Cat-No.ItemSizePrice
SRBAF488-1-10alpaca anti rabbit IgG, recombinant VHH, Alexa Fluor 48810 uL124.00
SRBAF488-1-100alpaca anti rabbit IgG, recombinant VHH, Alexa Fluor 488100 uL382.00
SRBAF568-1-10alpaca anti rabbit IgG, recombinant VHH, Alexa Fluor 56810 uL124.00
SRBAF568-1-100alpaca anti rabbit IgG, recombinant VHH, Alexa Fluor 568100 uL382.00
SRBAF647-1-10alpaca anti rabbit IgG, recombinant VHH, Alexa Fluor 64710 uL124.00
SRBAF647-1-100alpaca anti rabbit IgG, recombinant VHH, Alexa Fluor 647100 uL382.00

Chromobodies®

ChromoTek's Chromobodies® are a novel species of extremely small intracellular functional antibodies. Chromobodies® are based on the antigen binding domain (VHH) derived from heavy chain antibodies of camelids genetically fused to a fluorescent protein (e.g.TagGFP or TagRFP from Evrogen). As opposed to conventional antibodies, these innovative fluorescent nanoprobes are suitable for real time analyses and can visualize endogenous cellular structures and processes in live cells.

In addition to stable cell lines, you can also obtain a Chromobody® plasmid, which allows you to create your own cell lines or transiently express a Chromobody®.

All Chromobody® binding domains are carefully selected to not interfere with endogenous protein function and offer the unique possibility to trace native proteins in living cells.

F2H® assay

Study protein-protein interactions (PPIs) in mammalian cells with ChromoTek's Fluorescent Two-Hybrid (F2H®) Technology in a fast and simple way by conventional fluorescence microscopy. The direct optical readout is optimal for high-content analysis (HCA) and screening for PPI inhibitors.

Advantages

  • Visualize and validate PPIs in the intracellular environment
  • Support biochemical PPI analysis by fluorescence imaging
  • Identify interacting protein domains
  • Monitor PPI dynamics in live cells upon treatments
  • Determine inhibition kinetics
  • Screen for compounds inhibiting or activating PPIs

Spot-Tag® and Spot-Trap®

The ChromoTek Spot-System is the first peptide-tag specific nanobody for universal capture and detection applications. It comprises Spot-Tag®, an inert 12 amino acid peptide-tag (PDRVRAVSHWSS), and the universal, rugged Spot-nanobody that specifically binds to Spot-tagged proteins with high affinity.

Image on the right: interaction of Spot-nanobody (green) with Spot peptide.

Spot-Tag® advantages

  • More robust & stable
  • Higher affinity
  • Less background
  • Less unspecific binding
  • Higher resolution

Spot-nanobody advantages

  • Less negatively charged
  • Short
  • Inert

ChromoTek's Spot-System can also be applied for CRISPR'ed Spot-Tags (see Brochures section for an application note).

In addition, ChromoTek provides a growing number of exceptionally specific conventional antibodies.