ChromoTek offers innovative research reagents based on Camelid antibodies: Our Nano-Traps are perfect tools for the rapid immuno- precipitation of proteins and their interaction partners with high specificity and efficiency. Our Boosters enhance the signal intensity of your targets in fluorescent microscopy. And our recently introduced Chromobodies® allow you to analyze endogenous proteins in living cells in real time. In addition, we provide a growing number of exceptionally specific conventional antibodies.
Last but not least, our new fluorescent 2-hybrid (F2H®) assay enables you to monitor reversible protein-protein interactions in a cellular system.
Nano-Traps are ideal for fast, reliable and efficient one-step immunoprecipitations. They pull down your proteins of interest (POI) and their interacting factors from cell extracts or organelles. Nano-Traps consist of a VHH, also termed nanobody, coupled to an immobilizing matrix (see Technology). You can use Nano-Traps for a multitude of biochemical analyses such as:
- Immunoprecipitation (IP) / Co-IP
- Mass spectrometry
- Enzyme activity measurements
- ChIP / RIP analysis
ChromoTek's Nano-Boosters are very small, highly specific binding proteins covalently coupled to bright and stable fluorescent dyes. These GFP-, RFP- and Vimentin- binding proteins are derived from single-domain alpaca antibody fragments termed VHHs or nanobodies.
- Higher image resolution
- Excellent performance in IF and super-resolution microscopy
- Stabilization, enhancement and reactivation of fluorescent signals
- Less than 2nm epitope-label displacement minimizes linkage error
- Monovalent, recombinant single domain antibody fragment from camelid
Nano-Boosters (also termed nanobody or VHH) are much smaller compared to conventional primary plus secondary antibodies (IgG complex). Due to their small size, VHHs are particular suitable for effective labelling with minimal fluorophore displacement for super-resolution microscopy.
ChromoTek's Chromobodies® are a novel species of extremely small intracellular functional antibodies. Chromobodies® are based on the antigen binding domain (VHH) derived from heavy chain antibodies of camelids genetically fused to a fluorescent protein (e.g.TagGFP or TagRFP from Evrogen). As opposed to conventional antibodies, these innovative fluorescent nanoprobes are suitable for real time analyses and can visualize endogenous cellular structures and processes in live cells.
In addition to stable cell lines, you can also obtain a Chromobody® plasmid which allows you to create your own cell lines or transiently express a Chromobody®.
All Chromobody® binding domains are carefully selected to not interfere with endogenous protein function and offer the unique possibility to trace native proteins in living cells.
Study protein-protein interactions (PPIs) in mammalian cells with our Fluorescent Two-Hybrid (F2H®) Technology in a fast and simple way by conventional fluorescence microscopy. The direct optical readout is optimal for high-content analysis (HCA) and screening for PPI inhibitors.
- visualize and validate PPIs in the intracellular environment
- support biochemical PPI analysis by fluorescence imaging
- identify interacting protein domains
- monitor PPI dynamics in live cells upon treatments,
- determine inhibition kinetics
- screen for compounds inhibiting or activating PPIs