Cell Biolabs manufactures cell-based assays, viral expression and purification reagents, oxidative stress assays and small GTPase kits and reagents. Cell Biolabs' unique, cutting-edge products are currently used around the world in the research laboratories of universities, government institutes, biotechnology and pharmaceutical companies.
- Cell-Based Assays
- Viral Expression and Purification Kits and Reagents
- Oxidative Stress Assays
- Metabolism Research Assays and Reagents
- Cell Signaling and Protein Biology
The CytoSelect™ BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection). Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.
Figure 1: Assay Principle for the CytoSelect™ BrdU Cell Proliferation ELISA Kit.
Figure 2: Serum Stimulation of Proliferation in HEK 293 Cells. Cells were plated overnight at 37ºC. Cells were then incubated in the presence or absence of 10% FBS for 24 hours, followed by treatment with 10 µM BrdU for 1 or 3 hours. Cells were tested for BrdU incorporation according to the assay protocol.
|CBA-251||BrdU cell proliferation ELISA kit||96 rxns|
Cell Biolabs’ CytoSelect™ Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of PCNA from nuclear and whole cell extracts. The kit detects PCNA from mouse, rat and human, and has a detection sensitivity limit of 12.5 ng/mLPCNA. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.
- Detection sensitivity limit of 12.5 ng/mLPCNA
- Detects PCNA from mouse, rat and human and provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples
|CBA-254||CytoSelect™ Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit||96 rxns|
Cell Biolabs’ CytoSelect™ Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then incubated with the proliferation reagent. Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.
The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.
Figure 1: Human HEK 293 Cell Density. HEK 293 cells were seeded in triplicate at various densities indicated above and allowed to grow for 24 hours. After adding the CytoSelect™ Cell Proliferation Assay Reagent, cells were then incubated for 6 hours at 37ºC and 5% CO2.
|CBA-250||Fluorimetric cell proliferation assay reagent||10 ml|