Active Motif - Enabling epigenetics research

About Active Motif

Active Motif is the industry leader in developing and delivering innovative tools to enable epigenetics and gene regulation research. They are committed to providing the highest quality products and superior service to serve the life science, clinical and pharmaceutical/drug discovery communities.

Active Motif offers a comprehensive portfolio of high-quality products such as antibodies, recombinant proteins, assay kits and more for studies on

  • Chromatin immunoprecipitation (CHIP)
  • Histone modifications and analysis
  • DNA methylation
  • Transcriptional gene regulation

Product offering

Antibodies

Active Motif is committed to providing the highest quality antibodies for studying epigenetics in the context of histone and DNA modifications. Their antibodies are manufactured in-house, where they undergo rigorous developmental and validating procedures to ensure their quality and performance. Their staff scientists have also validated these antibodies for use in the applications such as chromatin immunoprecipitation (ChIP), ChIP-Seq, Western blot, and immunofluorescence.

Recombinant proteins

Active Motifoffers a large collection of full-length, high quality recombinant proteins for many relevant drug targets. Their selection also include different histone substrates. The proteins are manufactured to meet the highest standards of purity and activity required for conventional drug discovery assays, such as HTRF, mass spectrometry, AlphaLISA and Alphascreen, and these assays are routinely incorporated into their quality control processes.

Chromatin immunoprecipitation (ChIP)

Active Motif offers various innovative products to make your ChIP work easier and more successful, e.g. the ChIP-IT® Express Kits. These kits eliminate some of the ChIP challenges by providing a streamlined protocol using protein G-coated magnetic beads, making it possible to perform ChIP in just 1 day. Kits that use either sonication or enzymatic digestion for preparing sheared chromatin are available.

Which ChIP kit should you choose? Check out the selection guide below for source material cultured cells (left), frozen/FFPE tissue or PBMCs (right).

Or look here for a product summary and workflow overview.

Histone modification and analysis

How genetic material is organized in the nucleus profoundly influences processes such as transcription. A large part of genome regulation involves histone modifications and chromatin structure. In order to facilitate research in this field, Active Motif offers many tools, e.g. histone purification kits and various assays including sensitive histone modification ELISAs which quantitate modifications on histone tails.

Other

Active Motif also offers many other products, such as the Nu.Q™ Total Assay Kit designed for the detection of global levels of cell-free circulating nucleosomes (cf-nucleosomes) in human serum in a high-throughput format.

They also offer tools for the analysis of transcriptional regulation, e.g. the LightSwitch™ Reporter System.