Cytoskeleton promotion: Kit and item 20% off
Buy one kit and at least one other item and get 20% discount on both! This offer includes the highly cited Activation Assay Kits used to measure the GTP-bound form of small G-proteins such as Ras and Rho, and items such as K-Ras mutated proteins and SOS1 GEF proteins.
Quote CYTO19-2. Valid from March 1st until April 15th, 2019.
Cytoskeleton is a leader in providing the scientific community with the most robust, accurate, and time-saving kits to measure Small GTP-binding protein (SmG) activation. SmGs are involved in regulating cell signaling pathways and impact a wide range of cellular processes, functions, and morphology. Cytoskeleton offers two formats.
The traditional pull-down format, in which affinity beads are incubated with the extract and then separated by centrifugation. The pelleted products are separated by SDSPAGE and blotted onto a membrane for Western analysis of the SmG of interest.
The G-LISA® format, a modified ELISA which has the affinity reagent permanently attached to the wells of a 96-well plate. The extract is incubated in the well, then washed, and finally probed with primary and secondary antibodies. The G-LISA® enables more sensitive applications, e.g. measurement in limited primary cell numbers and Matrigel 3D matrices.
Kits exist for different SmGs, such as Rho, Rac, Arf1 & 6, Ras, Cdc42, and Ral.
Which Activation Assay is the right one for you? Find out in this video
|BK150||RhoA ELISA Kit||96 assays||713.00|
|BK035||Rac1 Pulldown Activation AssayKit||50 assays||1'026.00|
|BK126||Rac1 G-LISA Activation Assay Kit||96 assays||1'265.00|
|BK100||GEF Exchange Assay||60-300 assays||951.00|
|BK105||GAP Assay Kit||80-160 assays||951.00|
|CN03-A||Rho activator II||3 x 20 ug||328.00|
|ACD03||Anti-Cdc42 Mouse Monoclonal Antibody||2 x 200 ul||504.00|