Cytoskeleton promotion: Kit and item 20% off

GEFs, small G-proteins, and Activation Assays special offer

Buy one kit and at least one other item and get 20% discount on both! This offer includes the highly cited Activation Assay Kits used to measure the GTP-bound form of small G-proteins such as Ras and Rho, and items such as K-Ras mutated proteins and SOS1 GEF proteins.

Full list of eligible products

Quote CYTO19-2. Valid from March 1st until April 15th, 2019.

Cytoskeleton's Activation Assay Kits

Cytoskeleton is a leader in providing the scientific community with the most robust, accurate, and time-saving kits to measure Small GTP-binding protein (SmG) activation. SmGs are involved in regulating cell signaling pathways and impact a wide range of cellular processes, functions, and morphology. Cytoskeleton offers two formats.

The traditional pull-down format, in which affinity beads are incubated with the extract and then separated by centrifugation. The pelleted products are separated by SDSPAGE and blotted onto a membrane for Western analysis of the SmG of interest.

The G-LISA® format, a modified ELISA which has the affinity reagent permanently attached to the wells of a 96-well plate. The extract is incubated in the well, then washed, and finally probed with primary and secondary antibodies. The G-LISA® enables more sensitive applications, e.g. measurement in limited primary cell numbers and Matrigel 3D matrices.

Kits exist for different SmGs, such as Rho, Rac, Arf1 & 6, Ras, Cdc42, and Ral.

Which Activation Assay is the right one for you? Find out in this video

Selection from eligible products list

BK150RhoA ELISA Kit96 assays713.00
BK035Rac1 Pulldown Activation AssayKit50 assays1'026.00
BK126Rac1 G-LISA Activation Assay Kit96 assays1'265.00
BK100GEF Exchange Assay60-300 assays951.00
BK105GAP Assay Kit80-160 assays951.00
CN03-ARho activator II3 x 20 ug328.00
ACD03Anti-Cdc42 Mouse Monoclonal Antibody2 x 200 ul504.00