Still using Digitonin to stabilize your membrane proteins?
Here are 5 reasons why you should switch to GDN
1. No risk of toxic byproducts
While digitonin is a natural product and can contain harmful byproducts such as digitoxin and digoxin, GDN is produced synthetically, without toxins.
2. No batch-to-batch variation
Again, as a synthetic product, GDN has the benefit of not varying between batches as digitonin does.
3. Lower CMC
GDN's critical micelle concentration is 18uM - way lower than digitonin's 0.25-0.5 mM!
4. Better water solubility
With ≥10% water-solubility at RT, GDN beats digitonin even here!
5. It’s cheaper!
For example, 500mg GDN costs CHF 168.- vs. over 300.- for digitonin!
Have we convinced you yet?
At LubioScience, we offer Anatrace’s high-quality GDN101 (≥98% purity by HPLC analysis) in multiple sizes between 500 mg and 100 g.
GDN101 is on stock with 24h delivery time for customers in Switzerland.
Literature for GDN
Description of GDN and performance comparison to DDM:
Some recent Cryo-EM studies using GDN instead of Digitonin:
- Koehl, A., et al. (2018) Nature 558(7711): 547–552.
- She, J., et al. (2018) Nature 556(7699), 130–134.
- Hartler, A. M., et al. (2019) Nat Struct Mol Biol. 26(1): 78–83.
- Walter, J. D., et al. (2019) eLife 8: e46986.
- Qian, H., et al. (2019) Nat Commun. 10: 2320.
- Shanmuganathan, V., et al. (2019) eLife 8: e46267.
- Singh, A. K., et al. (2018) Nat Struct Mol Biol. 25(9): 805–813.