Synthetic genes

Custom genes for synthetic biology and other molecular biology applications

IDT's high-fidelity synthetic genes and gene fragments are the result of a long-standing commitment to innovative chemistry, optimized methods, and a robust manufacturing infrastructure. These products are built using their Ultramer® DNA Oligos, which set the standard for quality within the industry, and every gene or gene fragment you receive shares or exceeds that consistently high quality.

gBlocks® synthetic genes

gBlocks® Gene Fragments are sequence-verified, double-stranded DNA fragments that ship in only a few working days for affordable and easy gene construction or modification, to use in applications such as antibody research and CRISPR-mediated genome editing, as qPCR standards, and more. gBlocks® synthetic genes are available in tubes or plates.

  • Save on reagent costs with constructs as low as half the price of synthetic genes
  • Industry-leading DNA synthesis methods ensure error-free genes
  • Start your projects quickly with shipping in as few as 2 business days
  • Sequence-verified, from 125 bp up to 3 kb long

Tips for working with gBlocks® Gene Fragments

Easy gene assembly—gBlocks® Gene Fragments

Special offer: receive one gBlock for free!

  • Go to the IDT ordering portal via the link below
  • Place your order for a gBlock
  • Hit "checkout" and register if you don't have an account yet
  • Enter the code "TryIDTgBlocks" into the Purchase Order (PO) field during checkout
  • You will receive your free gBlock within 3-5 days

Order your free gBlock now

Megamer® single-stranded DNA fragments

Megamer® single-stranded DNA fragments are sequence-verified, single-stranded DNA strands of length 201–2000 bases. They can be used in applications such as homology-directed repair of CRISPR-mediated genome editing, in vitro transcription, and more.

  • Generated from clonally purified DNA, which offers the greatest purity available
  • Sequence-verified via next generation sequencing
  • Typically shipped within 20 business days

Megamers® in CRISPR applications

One-step strategy for KO mice

Order Megamers® at IDT

Ultramer® DNA oligonucleotides

Ultramers® are long, high-quality oligos for demanding applications such as cloning, ddRNAi, homology-directed repair, and gene construction.

Ultramer® DNA oligonucleotides are generated by proprietary synthesis methods that deliver high quality oligos up to 200 bases. They are available single- or double-stranded and can be delivered in tubes or plates.

  • Highest possible quality thanks to industry-leading synthesis pipeline
  • Complete confidence in oligos that are verified by electrospray ionization-mass spectrometry
  • Unrivalled control of oligo specifications with custom formulation and mixing options

Getting enough full-length oligo?

Order Ultramers® at IDT

Integrated DNA Technologies - the industry leader in DNA synthesis

IDT's proprietary DNA synthesis equipment permits rapid, high quality synthesis of nucleic acids. This platform is adjusted to a lower throughput mode which uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, Ultramer® DNA oligos use a unique solid support that is specifically optimized to synthesize low-yield, high-quality oligos up to 200 bases in length. Overall, these improvements to their already established manufacturing methods allow you to acquire longer, purer oligos for your research.

Full-length product received is determined by coupling efficiency

Ultramer® DNA oligos are manufactured on proprietary synthesis platforms that offer higher coupling efficiencies than IDT's standard DNA oligos and other industry standards. As seen in the graph, coupling efficiency becomes increasingly important as oligo length increases. IDT Ultramers® achieve significantly higher coupling efficencies than competitors like Eurofins, Geneart or Genscript.