DNA Library Prep Kits

Vertasile offering of NGS library prep kits

We have a versatile library prep kit offering for almost every application and need. Check them out below and contact us if you have any question.

Lotus DNA library prep kit

One kit. Flexible workflow. Endless applications.

One kit. Flexible workflow. Endless applications.

The Lotus DNA Library Prep Kit enables streamlined preparation of high-quality next generation sequencing (NGS) libraries from double-stranded DNA (dsDNA). The kit uses enzymatic fragmentation to generate libraries suitable for PCR-free, PCR-amplified, and targeted sequencing applications on Illumina platforms.

  • Get the uniform sample coverage you need without relying on expensive equipment
  • Regain valuable time with a fast, simple workflow
  • Create application-specific NGS libraries by adding IDT adapters and xGen products for target capture

The Lotus kit can be customized for your applications when combined with one of the many IDT adapter options (see Ordering section). Additionally, use of xGen hybridization capture products provides a complete NGS solution that takes you from sample preparation to sequencing.

Swift 2S Turbo DNA library kits

The Easiest NGS Workflow for Routine Sequencing

Swift 2S Turbo DNA Library Kits are a fast, efficient and cost-effective library prep solution. Leveraging a robust enzymatic fragmentation prep and flexible indexing, you can prepare high-quality whole genome and exome libraries using a broad range of input amounts and sample types.

  • High-throughput: Quick 2-hour workflow, 768-indexing, and robust fragmentation.
  • Automation ready: Scripts available for Beckman, Hamilton and PerkinElmer. Fast-track help available to assist with automating on your platform.
  • Budget friendly: >30% per sample over similar products on the market.
Swift 2S Turbo DNA Library Kits come in two configurations:

2S Turbo: All-in-one for quick implementation

Whether discovering or screening germline mutations, 2S Turbo makes library prep more accessible to any laboratory. This kit configuration includes library prep reagents and Swift adapter compatible with several indexing options such a Single, Combinatorial Dual (up to 768-plex) or Unique Dual Indexing.

2S Turbo Flexible: Broaden the range. Expand your scale.

Need a powerful pipeline to detect any variant across any sample quality and quantity? Combine our 2S Turbo Flexible with your choice of full-length indexed adapters (e.g. Illumina TruSeq DNA Single Indexes and TruSeq DNA CD, etc..) to create a fast, efficient, and scalable workflow to detect both rare and common variants in any size project.

“Wanted to add, I am really impressed with the uniformity in the library size! Haven’t seen that ever with a transposase based library kit.”-From a Client in Maryland 

“We spoke a few weeks ago on a tech support call regarding using the Swift 2S Turbo Library Prep Kit on a mixed microbial plasmid DNA input. I wanted to let you know we followed the 10ng input parameters and it worked beautifully! Our final libraries were slightly large (~450-500bp) but still gave us high-quality sequencing reads and great data. Thank you for your help!”-Elinne Becket, Ph.D., Assistant Professor-Department of Biological Sciences, California State University, San Marcos

Swift 2S Turbo in our shop

Accel-NGS 2S DNA library kits

The Accel-NGS 2S family of products employ a combination of unique fragment repair mechanisms and an exceptionally efficient adapter technology to generate the most diverse libraries for Illumina® platforms. The synergy in this process allows for processing DNA inputs as low as 10 pg while minimizing base composition bias in final libraries. All of the Accel-NGS 2S Kits permit for single-tube processing of just a few samples with a simple protocol while easily being automated for high-throughput labs.

The Accel-NGS 2S family is the best choice for users seeking the most versatile, highest performing library prep kit for double-stranded DNA inputs, including ChIP-Seq, cfDNA, and FFPE samples. Expand your capabilities, run those difficult samples, or add new services to a core lab. With the Accel-NGS 2S DNA Library Kits, you can expand your sequencing capability and construct libraries from limiting DNA samples.

ACCEL-NGS 2S PRODUCTS:

Accel-NGS 2S Hyb DNA Library Kit

NGS Prep for Hybridization Capture

Minimize duplicates, maximize sequencing data

Accel-NGS 2S Plus DNA Library Kit

Versatile DNA Library Preparation for Next-Generation Sequencing

Improve DNA sequencing results by covering more of the genome

Accel-NGS 2S PCR-Free DNA Library Kit

PCR-free NGS Prep with Low Input Capability 

Eliminate sequencing bias from polymerase with PCR-free preps

Accel-NGS 2S in our shop

Accel-NGS Methyl-Seq library kits

Low-input Methylation Sequencing

The Accel-NGS Methyl-Seq DNA Library prep utilizes Adaptase® technology for capturing single-stranded DNA molecules in an unbiased manner. As bisulfite treatment of samples damages DNA, Swift Biosciences’ single-strand compatible Adaptase technology maximizes recovery of DNA from bisulfite-converted samples. The resultant libraries accurately represent sample base composition of the entire genome for the analyzed organism. The technology has quickly become the gold standard for providing the most comprehensive coverage of the methylome. It is an excellent choice for whole genome bisulfite sequencing as well as targeted bisulfite sequencing applications, such as RRBS and hybridization capture using the NimbleGen™ SeqCap™ Epi Enrichment Sysem. The Accel-NGS Methyl-Seq Kit is also compatible with bisulfite-converted DNA samples enriched by ChIP or other methods. This library preparation technology contains a uracil tolerant polymerase which is ideal for sequencing ancient DNA samples that may contain uracil nucleotides as a result of damage.

ACCEL-NGS METHYL-SEQ PRODUCTS:

Accel-NGS Methyl-Seq DNA Library Kit

The gold standard for single-base resolution of methylomes

Accel-NGS Adaptase Module

Prepare Single-Cell Methyl-Seq Libraries

Accel-NGS Methyl-Seq in our shop

Accel-NGS 1S DNA library kits

NGS DNA Library Construction from Samples with Limiting Amounts of Degraded DNA

The Accel-NGS 1S DNA Library Kits enable users to make libraries from the impossible. Libraries can be made from precious, damaged and degraded samples by using Swift’s Adaptase™ technology. Unlike other library methods, the Adaptase technology can generate library molecules from single-stranded DNA fragments, which allow researchers to recover more of their input DNA from difficult and rare samples compared to other commercially-available products-for Ilumina® NGS Platforms

The Accel-NGS 1S Library Kits will yield libraries in 2 hours from as little as 10 pg of input and fragments as short as 40 bp. The kits work with precious and difficult-to-use samples, such as single-stranded DNA, heat-denatured pathogenic samples (microbial DNA), viral DNA, first-strand cDNA, ancient DNA, ChIP-Seq, and degraded DNA from FFPE samples. Accel-NGS 1S Library Kits are the best choice for users needing to sequence treasured or difficult-to-process samples, which cannot be sequenced by other methods. Expand your research by processing precious, damaged and degraded samples on either the Illumina® or Ion Torrent™ NGS platforms.

ACCEL-NGS 1S PRODUCTS:

Accel-NGS 1S Plus DNA Library Kits

DNA Library Preparation of Difficult Samples 

Rescue valuable sequencing data from precious samples

Accel-NGS DNA Library Kit for Ion Torrent™

DNA Library Preparation for NGS on Ion Torrent Platforms

Save time with a simple, 2-hour prep for Ion Torrent

“We choose the Swift 1S kit for construction of sequencing libraries when available sample is low, the quality is uncertain, or when we want to capture single stranded as well as double stranded DNA. We have found the kit easy to use with well labeled components and a straightforward protocol. We find that libraries rarely fail with this kit even when starting material is highly degraded. The availability of unique dual indexes is also key to allow sequencing on any available Illumina platform. With regards the samples from the NASA Twins study, we were most interested in being able to detect single-stranded DNA viruses as well as all other organisms as part of our shotgun metagenome sequencing project, and this drove the decision to use the Swift 1S kit.”

Stefan J Green, Ph.D. Director, Sequencing Core, Associate Director, RRC and
Kevin Kunstman, MS Assistant Director Sequencing Core, University of Illinois at Chicago

“I would like to give a summary that we tried to use a competitor’s kit first for our project but it ended up that we needed to do 17 PCR cycles to achieve the concentration of libraries that we needed. But what that meant was that there would be increased duplication rate when we did next-generation sequencing. With your Swift 1S we only needed to do 4-5 PCR cycles and our duplication rate was 7-13% when using HiSeq4000 for deep sequencing. We were interested in both ssDNA and dsDNA. We are happy with your product.”

Premi Haynes Ph.D.
Senior Fellow in the Laboratory of Dr. Daniel Miller
University of Washington

Accel-NGS 1S in our shop

NxSeq® AmpFREE low DNA library kits

The highest efficiency, low input, pcr-free fragment library prep kit available at the lowest cost.

  • Low Input: Requires as little as 75 ng of sheared input DNA allowing use of limiting samples.
  • High Efficiency: Optimized adaptor ligationproduces more sequenceable fragments in each library, yielding better coverage & depth from single or multiplexed libraries.
  • PCR-free: Prevents the introduction of PCR-bias, providing more uniform coverage.
  • Fast: 2 hour, 10 minute protocol saves you time and gets your samples on the sequencer sooner.
  • Affordable: Best priced and best performing kit available.

Product Offering

Cat-No.ItemSizePrice
14000-1NxSeq AmpFREE Low DNA Library Kit, 12 Reactions12 reactions359.00
14000-2NxSeq AmpFREE Low DNA Library Kit, 48 Reactions48 reactions1'233.00
14300-1NxSeq Adaptors, Box 1 (12 x 4 Reactions)12 x 4 reactions295.00
14400-1NxSeq Adaptors, Box 2 (12 x 4 Reactions)12 x 4 reactions295.00

NxSeq® UltraLow DNA library kit

Lowest input, highest efficiency, Illumina-compatible DNA fragment library prep kit

  • High Quality Data: High efficiency adaptor ligation produces complex libraries that yield improved sequencing depth uniformity and better coverage with fewer zero coverage regions
  • Sensitive: Construct DNA fragment libraries from as little as 50 pg to as much 75 ng of sheared/fragmented DNA
  • Minimal Bias: Robust, uniform PCR amplification improves coverage uniformity when working with low input amounts of genomic DNA
  • Flexible:Extensively tested in de novo whole genome sequencing or resequencing, but compatible with other applications such as exome-seq, ChIP-seq and FFPE DNA samples.
  • Fast: 3 hour protocol gets your samples on the sequencer quicker
  • High Value: Cost-effective library and indexing kits which produce excellent sequencing results