Hybridization Capture

xGen exome research panel

Deep, uniform coverage of the human exome with best-in-class capture panel

The xGen Exome Research Panel v1.0 consists of 429,826 individually synthesized and quality controlled xGen Lockdown Probes. The Exome Research Panel spans a 39 Mb target region (19,396 genes) of the human genome and covers 51 Mb of end-to-end tiled probe space. All probes in the panel are manufactured using GMP standards. Mass spectrometry and OD measurements are taken for each probe to ensure appropriate representation of the correctly manufactured probes in the pool.

  • Obtain consistent results with individually synthesized target capture probes manufactured to GMP standards
  • Achieve deep and uniform coverage even across difficult GC-rich regions
  • Integrate with common sequencing platform

NGS discovery pools

Days vs. weeks might mean everything

NGS Discovery Pools are high-fidelity, individually-synthesized, 5′-biotinylated oligos for targeted NGS. Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. These nucleic acid probes can be used for creating custom capture panels that can be optimized, expanded, and combined with other panels. Improvements to our established manufacturing process allow you to build custom panels quickly at a fraction of the cost of conventional custom panels.

  • Fastest custom panel turnaround time in the industry
  • Affordable for any size panel
  • Easy design for even coverage across your target

xGen lockdown probes pool

Custom target enrichment panels for maximum flexibility and performance

xGen Lockdown Probe Pools contain individually synthesized Lockdown Probes pre-formulated to deliver consistent, convenient, best-in-class performance.

  • Achieve higher performance with individually synthesized and quality-controlled capture probes
  • Develop cost-effective custom panels that are delivered quickly (7–10 business days for orders <2000 probes)
  • Optimize, expand, or combine custom panels

xGen predesigned gene capture pools

Preconfigured probe pools designed to target human gene coding sequences for cost-effective custom NGS panels.

xGen Predesigned Gene Capture Pools contain individually synthesized Lockdown Probes pre-formulated into individual gene pools for easy selection and setup of custom target panels.

  • Generate deep and uniform coverage data through superior hybridization capture performance
  • Choose only the genes you want, delivered quickly with a choice of formats
  • Obtain full exon coverage for a given gene with each gene pool design

xGen lockdown panels

xGen Lockdown Panels are stocked enrichment panels for targeted next generation sequencing. The panels consist of individually synthesized and quality-controlled xGen Lockdown Probes that have been validated to provide the highest level of performance.

xGen Lockdown Panels

xGen Lockdown Panels are stocked enrichment panels for targeted next generation sequencing. The panels consist of individually synthesized and quality-controlled xGen Lockdown Probes that have been validated to provide the highest level of performance.

xGen Exome Research Panel »

Exome capture panel manufactured using GMP standards targeting 19,396 genes for comprehensive, uniform coverage of human coding regions.

xGen Acute Myeloid Leukemia Cancer Panel »

Predesigned panel targeting >260 genes associated with AML for more efficient detection of disease-causing mutations.

xGen Pan-Cancer Panel »

Predesigned panel that captures 127 significantly mutated genes implicated across 12 tumor types for deeper sequencing coverage.

xGen Inherited Diseases Panel »

Predesigned panel for targeted enrichment of genes and SNPs associated with inherited diseases.

xGen Human ID Research Panel »

Predesigned spike-in panel for targeted enrichment of a definitive set of single nucleotide polymorphisms (SNPs) selected to identify a unique individual from a population of over 5 million biological samples.

xGen Human mtDNA Research Panel »

Predesigned spike-in panel for cost-effective and accurate detection of sequence variants in mitochondrial DNA.

xGen CNV Backbone Panel—Tech Access »

Predesigned spike-in panel that can be used to identify copy number variations in human NGS data.

xGen blocking oligos

Improve on-target performance during targeted sequencing by reducing adapter-to-adapter hybridization

xGen Blocking Oligos bind to library adapter sequences to reduce off-target capture during library enrichment. Choose from convenient, optimized, predesigned mixes or build your own custom blocking oligo. xGen Universal Blockers are designed for use with Illumina indexed adapters. These ready-to-use mixes effectively block dual- or single-index adapters to significantly improve on-target performance.

  • Reduce sequencing cost per sample by improving on-target performance
  • Effectively block a variety of index adapter designs with proprietary sequence modifications
  • Eliminate complicated blocker selection by using worry-free formulations compatible with library prep kits, such as Illumina’s ligation-based TruSeq® kit or Illumina’s Nextera® kit
  • Reduce workflow steps with a convenient premixed liquid formulation

Custom xGen Blocking Oligos are also available for other platforms, including Ion Torrent, and specialized applications.

xGen hybridization ans wash kit

Optimized reagents for the xGen hybridization capture workflow

The xGen Hybridization and Wash Kit comprises buffers, Cot DNA, and streptavidin beads required for hybridization capture of DNA. The kit has been developed to work with xGen Lockdown Probes and Panels and xGen Blocking Oligos for a complete, high quality target enrichment solution. This workflow is compatible with NGS libraries prepared using ligation-based techniques, such as TruSeq® library kits, and Nextera® DNA library preparation kits.

  • Achieve uniform coverage and robust capture performance across a broad range of xGen hybridization probe panels
  • Generate highly reproducible targeted NGS data with quick and easy workflow results
  • Updated, automation-friendly protocol enables various levels of throughput