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PrimeTime qPCR probes, primers and assays from IDT

PrimeTime® qPCR probe assays

Primer and probe premixed assays for analyzing gene expression in any species using 5′ nuclease fluorescent probes

PrimeTime qPCR Probe Assays consist of a primer pair and 5′ nuclease probe. Predesigned assays for human, mouse, or rat are available for easy selection based on multiple criteria such as exon location and number of transcripts detected. Custom assays may be created for any sequence from any species using the PrimerQuest® tool.

  • Achieve >90% efficiency over 4 or more orders of magnitude with our predesigned assays, guaranteed
  • Multiplex with confidence with double-quenched probe assays that have the lowest background in the industry
  • Adjust your primer probe ratio from 2:1 to 4:1 with no additional charge
  • Receive primers and probe sequences with all orders

Visit the IDT website for more information

PrimeTime® qPCR primer assays

Primer-only premixed assays for analyzing gene expression using intercalating dyes

PrimeTime qPCR Primer Assays provide a primer pair designed for real-time PCR using intercalating dyes, such as SYBR® Green (Molecular Probes) or EvaGreen® (Biotium) dyes. Predesigned assays for human, mouse, or rat are designed with advanced bioinformatic and thermodynamic sequence analytics and for easy selection. Custom assays may be created for any sequence from any species using the PrimerQuest® tool.

  • Advanced bioinformatics delivers the best design possible, with considerations for exon location and number of transcripts detected
  • Achieve >90% efficiency over 4 or more orders of magnitude with our predesigned assays, guaranteed
  • Test target sequence amplification prior to using more expensive probe-based assays
  • Receive primer sequences with all orders

Visit the IDT website for more information

PrimeTime® gene expression master mix

Achieve quality performance under various experimental conditions with this thermostable probe-based qPCR master mix

PrimeTime Gene Expression Master Mix is a 2X solution containing a hot-start antibody, Taq polymerase, and other components needed for probe-based qPCR in two-step RT-PCR experiments. Reference dye is included separately.

  • Achieve high efficiency qPCR with fast or standard cycling
  • Use one mix for singleplex or multiplex reactions
  • Rely on exceptional benchtop stability to obtain consistent results from overnight experiments
  • Explore license-free options for commercial or diagnostic use

Visit the IDT website for more information

PrimeTime® qPCR 5′ nuclease probes

Generate reliable, high-quality gene expression analysis data with double- and single-quenched fluorescent hydrolysis probes

PrimeTime qPCR Probes are 5′ nuclease probes, available with an assortment of reporter-dye combinations that are compatible with common qPCR instruments and that include several license-free combinations.

  • Reduce background signals, even with longer probes, when using ZEN™ or TAO™ Double-Quenched Probes
  • Successfully multiplex with reduced crosstalk using double-quenched probes
  • Achieve high efficiency of qPCR under fast or standard cycling conditions
  • Minimize troubleshooting: PrimeTime qPCR Probes are verified by mass spectrometry and HPLC-purified

Visit the IDT website for more information

Special offer - receive a qPCR assay for free!

Quote "MINIME" to receive a predesigned qPCR assay, mini format (100 reactions). Valid until December 31, 2018 for new IDT customers. 

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