DNA purification

DNA purification kits by Norgen

  • DNA isolation kits for all types of samples
  • Purified DNA is of the highest quality and integrity for sensitive downstream applications incl. PCR, qPCR, genotyping, sequencing and more
  • DNA kits for gDNA, cell, bacteria, yeast, plants, tissue, FFPE, soil, saliva, urine, blood and plasma, and many more
  • Available in spin column format, 96-well plates, and magnetic beads

Norgen’s Magnetic Bead System: Magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions.

Genomic DNA isolation

Genomic DNA Isolation Kit

This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 min (including incubation time).

Norgen also offers a kit for sequential DNA, RNA and protein isolation from one sample (RNA/DNA/Protein Purification Plus Kit).

Cat.-No.Product nameSize 
24700Genomic DNA Isolation Kit50 prepsDatasheet
24750Genomic DNA Isolation Kit100 prepsDatasheet

 

Cells and Tissue DNA Isolation Kit

This kit is designed for the isolation of genomic DNA from cultured cells, viruses and various tissue samples.

  • Rapid spin column format
  • High yield
  • Excellent quality
  • DNA ready for any application including PCR, qPCR, genotyping and more
  • Protocol completed in approx. 30 minutes for cells or 90 minutes for tissues

For small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM) samples, Norgen has designed the Cells and Tissue DNA Isolation Micro Kit. The genomic DNA is preferentially purified from other cellular proteinaceous component and is eluted into a small elution volume of 20–40 μl. Typical yields of genomic DNA will vary depending on the sample being processed. Preparation time for a single sample is approximately 60 minutes.

Norgen also offers a cell and tissue DNA purification kit based on the use of magnetic beads. This Cells and Tissue DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for purifying DNA of the highest quality which is compatible with a number of downstream research applications including PCR, NGS and microarray analysis. gDNA can be isolated from cultured cells as well as various tissue samples, cultured cells, bodily fluids and nasal or throat swabs. This kit is also available in a 96-well format for high throughput applications.

Cat.-No.Product nameSize 
53100Cells and Tissue DNA Isolation Kit50 prepsDatasheet
57300Cells and Tissue DNA Isolation Micro Kit50 preps 
59100Cells and Tissue DNA Isolation Kit
(Magnetic Bead System)
50 preps 
62500Cells and Tissue DNA Isolation 96-Well Kit
(Magnetic Bead System)
2x 96-well plates 

 

DNA isolation from special samples

FFPE DNA Purification Kit

This kit was developed to extract and purify DNA from formalin-fixed paraffin-embedded (FFPE) samples with a fast and easy processing using convenient spin-columns.

The use of formalin in tissue fixation leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, SNP and STR genotyping. The protocol can be completed in as little as 1 hour.

Norgen also offer a special kit for the sequential isolation and purification of total RNA and genomic DNA.

Cat.-No.Product nameSize 
47400FFPE DNA Purification Kit50 prepsDatasheet
54300FFPE RNA/DNA Purification Plus Kit50 prepsDatasheet

 

Blood DNA Isolation Kits

Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. For larger input volumes of whole blood, Norgen offers a Midi (0.3 to 2 ml) and a Maxi (3 to 10 ml) kit.

  • Isolate genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA
  • Works with blood of various species, including humans
  • High yield and high quality DNA ready for any application, incl. PCR, qPCR, sequencing, genotyping, virus detection, and southern blot analysis

Norgen also offers a Blood DNA Isolation Kit with Magnetic Bead System. This kit is designed for the rapid preparation of genomic DNA from up to 200 µl of whole blood from various species, incl. human, using magnetic beads as the separation matrix. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.

For the isolation of genomic DNA from dried blood spots of anticoagulated and untreated blood, use the Dried Blood Spot DNA Isolation Kit. The purified DNA is of high quality, completely free from RNA contamination, and is completely compatible with downstream applications including PCR, qPCR and more.

Cat.-No.Product nameSize 
46300/
46380
Blood DNA Isolation Mini Kit50 preps/
100 preps
Datasheet
51400Blood DNA Isolation Midi Kit20 prepsDatasheet
31200Blood DNA Isolation Maxi Kit12 prepsDatasheet
46350Blood DNA Isolation 96-Well Kit2x 96-well platesDatasheet
59800Blood DNA Isolation Kit
(Magnetic Bead System)
50 preps 
62600Blood DNA Isolation 96-Well Kit
(Magnetic Bead System)
2x 96-well plates 
36000Dried Blood Spot (DBS) DNA Isolation Kit50 prepsDatasheet

 

Plasma/Serum Cell-Free Circulating DNA Purification Kits

This kit rapidly and simply purifies all sizes of circulating DNA (cfc-DNA) from plasma and serum samples. The plasma/serum input volumes are very versatile, ranging from 10–200µl for the Micro Kit, 200–500 µl for the Mini Kit, 1–4ml for the Midi Kit, and 5–10ml for the Maxi Kit. The latter two kits for larger volumes purify and concentrate DNA using a convenient two column system.

  • Also isolate viral and bacterial DNA
  • Concentrate circulating DNA into a flexible elution volume ranging from (25–50µl)
  • Isolate inhibitor-free cell-free circulating DNA
  • Purify high-quality DNA in 15–20 min for Micro and Mini Kit, 90 min for Midi and Maxi Kit
  • Compatible with Streck Cell-Free DNA BCT® Tubes
Cat.-No.Product nameSize 
55500Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit50 prepsDatasheet
55100Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit50 prepsDatasheet
55600Plasma/Serum Cell-Free Circulating DNA Purification Midi Kit20 prepsDatasheet
55800Plasma/Serum Cell-Free Circulating DNA Purification Maxi Kit10 prepsDatasheet

 

Plasmid DNA Prep Kits

The Plasmid MiniPrep Kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns.

  • High yield of plasmid DNA (typically up to 20 μg from 1.5 ml of bacterial culture)
  • Purify plasmids up to 13 kb
  • Plasmid DNA is ready for restriction digestion, bacterial transformation, sequencing and more

Also available in Maxiprep format (input up to 100 ml,  0.4 -1.0 mg yield from 100ml culture for high copy number plasmid)

Norgen also offers a Plasmid MiniPrep Kit (Magnetic Bead System) designed for the rapid preparation and isolation of high quality plasmid DNA from small batch cultures of Escherichia coli using their magnetic bead matrix. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS. This kit is also available in a 96-well format.

The BAC DNA MiniPrep Kit is designed for the rapid preparation of BACs (bacterial artificial chromosomes) and other large DNA constructs from small batch cultures of Escherichia coli. Rapid alcohol precipitation or spin-column purification allows for the processing of multiple samples in 1 hour.​ Typical DNA recoveries range between 0.6 µg and 1 µg from 3.0 ml of bacterial cultures. The purified BAC DNA is fully digestible with all restriction enzymes tested, and is fully compatible with bacterial transformation and sequencing to achieve 95-100% accuracy.

Cat.-No.Product nameSize 
13300/
46400
Plasmid MiniPrep Kit50 preps/
250 preps
Datasheet
46500/
46600
Plasmid DNA MaxiPrep Kit4 preps/
20 preps
Datasheet
60300Plasmid MiniPrep Kit
(Magnetic Bead System)
50 preps 
63000Plasmid MiniPrep 96-Well Kit
(Magnetic Bead System)
2x 96-well plates 
18050BAC DNA MiniPrep Kit50 preps
(1.5 ml)
Datasheet

 

Endotoxin removal

Endotoxin Removal Kit

This kit quickly removes endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. The typical recovery of DNA is >90% of the starting sample. Three formats are available for different amounts of input DNA: Mini (25 µg), Midi (200 µg) and Maxi (1 mg).

  • Reduce endotoxin levels to 0.1 EU/µg DNA or less
  • Effectively remove endotoxins in as little as 20 minutes (Mini format) or 30 min (Midi and Maxi formats)
  • Rapid spin-column format
Cat.-No.Product nameSize 
22700Endotoxin Removal Kit (Mini) - For DNA25 prepsDatasheet
52200Endotoxin Removal Kit (Midi) - For DNA10 prepsDatasheet
21900Endotoxin Removal Kit (Maxi) - For DNA4 prepsDatasheet

 

DNA clean-up kits

PCR Purification Kit

This kit quickly purifies amplified DNA products from PCR mixes using either spin-columns or 96-well plates for column chromatography.

  • Purify amplified DNA ranging from 100–15’000 bp in size
  • Effectively remove PCR by-products incl. primers, dimers, enzymes, unincorporated nucleotides and mineral oil
  • Purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more
  • Can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.
Cat.-No.Product nameSize 
14400PCR Purification Kit50 prepsDatasheet
45700PCR Purification Kit250 prepsDatasheet
24800PCR Purification 96-well Kit2x 96-well plateDatasheet

 

DNA Gel Extraction Kit

The DNA Gel Extraction Kit quickly (<20 min) and easily extracts and purifies DNA from agarose gel fragments using spin columns. The recovered DNA is free from agarose and other impurities, and is compatible with restriction enzyme digestion, ligation into vectors and sequencing.

Cat.-No.Product nameSize 
13100DNA Gel Extraction Kit50 prepsDatasheet

NGS Normalization 96-Well Kit

This kit allows for high-throughput PCR amplicon clean-up and normalization of PCR product concentration (~5 ng/µl). Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product.

  • Simultaneously clean-up and normalize PCR products
  • Remove all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides
  • Fast (less than 20 minutes), high-throughput and easy processing using centrifugation
  • Sufficient elution volume (100 µl) for repeat or future assays
  • Non-magnetic bead purification
Cat.-No.Product nameSize 
61900NGS Normalization 96-Well Kit2x 96-well platesProtocol

 

You can also find the whole list of available products in our shop.