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CRISPR-Cas9

Alt-R® CRISPR-Cas9 genome editing - point, click, edit. Guaranteed.

The Alt-R® CRISPR-Cas9 System includes all of the reagents needed for successful genome editing based on the natural S. pyogenes CRISPR-Cas9 system. Discover what makes the Alt-R CRISPR-Cas9 system best in class.

  • Benefit from the latest improvements in on- and off-target design and chemical modifications, as well as easyordering of custom or predesigned guide RNAs.
  • Get optimal editing with high on-target potency and reduced off-target activity with Alt-R HiFi CRISPR-Cas9 nuclease
  • Precisely control editing with efficient delivery of the RNP by lipofection or electroporation
  • Protect your cells from toxicity or innate immune response activation

Start CRISPRing the easy way - with our CRISPR/Cas9 starter kit!

The CRISPR-Cas9 starter kit from IDT supplies all reagents necessary for a complete genome editing experiment.

Kit ItemOrder #AmountDescriptionPrice
(CHF)

2 crRNAs

Custom2 nmole eachTwo custom crRNAs targeting two different loci adjacent to a PAM site in your gene of interest

220.50

tracrRNA

10725325 nmoleThe tracrRNA assembles together with a custom cRNA and the Cas9 protein into the RNP complex

110.25

Cas9 nuclease

1074181
1078727
 100 μg Standard or HiFi Cas9 enzyme, part of the RNP complex

225.75

Electroporation
enhancer

10759152 nmoleEnhances efficiency when introducing the RNA complex into target cells via electroporation

52.50

CRISPR-Cas9
control kit

1072554
1072555
1072556
1 kit1 kit Separate kits for human, mouse, or rat. Includes tracrRNA, HPRT positive control crRNA, negative control crRNA#1, HPRT Primer Mix and Nuclease-Free Duplex Buffer

173.25

Genome editing
detection kit

1075931

1 kit

(25 rxns)

For detection of on-target or known off-target CRISPR events in cultured cells. The T7 endonuclease I (T7EI) mismatch cleavage assay detects on-target genome editing and estimates genome editing efficiency in CRISPR treated cells

110.25

IDTE buffer,
pH 7.5

11-01-02-0210 x 2 ml1x TE buffer pH 7.5, nuclease-free, sterile, for resuspension of RNP complexes

20.85

Total

   

913.35

Introductory offer: 5% off

Buy a CRISPR starter kit now and receive 5% off all components.

Quote "StartCRISPR". Valid untl December 31, 2018 for new IDT customers.

Contact us now

CRISPR-Cas9 tutorials

Optimized methods to use Cas9 nickases in genome editing

HiFi Cas9 nuclease

Alt-R S.p. HiFi Cas9 Nuclease V3 offers improved specificity over wild-type Cas9, greatly reducing the risk of off-target cutting events. This Cas9 variant also preserves the high level of editing efficiency expected from a Cas9 nuclease, maintaining 90–100% on-target editing activity at most sites. For applications that are sensitive to off-target events, combining the Alt-R S.p. HiFi Cas9 Nuclease V3 with the optimized Alt-R CRISPR-Cas9 gRNA (crRNA:tracrRNA) is highly recommended.

Read the paper!

A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells. Vakulskas C, Dever D, et al. Nature Medicine. doi: 10.1038/s41591-018-0137-0 (2018)

Choose the right enzyme for your CRISPR experiment

How to order CRISPR-Cas9 reagents

To order the CRISPR-Cas9 starter kit or individual CRISPR-Cas9 reagents:

  • Directly order on the IDT portal by creating a new account 
  • Contact us via the "Ask for a quote" button below and we will assist you with ordering