10 Tips for NGS Library Preparation
Don't forget to spin down the vial before opening. This ensures everything is at the bottom of the vial and not in a cap or around the slides.
Make sure you use filter tips to minimize contamination.
Make sure you clean your working surface with DNAZap products and make sure you change your gloves frequently.
Use good pipetting technique. To efficiently mix reaction components, gently pipette up and down and do not vortex.
Always make a mastermix. Thaw and keep all master mixes on ice to ensure optimal activity.
When mixing reagents before addition to your reaction, keep everything on ice immediately after mixing.
Take care with your bead-based cleanups. Always use freshly prepared ethanol solutions for bead washes.
Be sure to return enzymes to the freezer after use to ensure product stability
To ensure the stability of your final library, store your final libraries in TE buffer and in low-bind DNA tubes.
Keep good records. Write down lot numbers of enzymes or kits and keep your notes if you're following a kit protocol