TOPO® TA Cloning® Kit for subcloning with TOP10 E. coli, 40 reactions

For fast, efficient subcloning in a variety of applications, the only choice is Invitrogen’s TOPO® TA cloning Kit with our popular pCR®2.1 vector. By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable method, taking only 5 minutes using a 3 step protocol and yielding up to 95% recombinants.

Convenient features of the TOPO® TA cloning Kit with our pCR2.1®-TOPO® TA vector are:3´-T overhangs for direct ligation of Taq-amplified PCR products
• T7 promoter and M13 forward and reverse primer sites for in vitro RNA transcription and sequencing
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• 15 Convenient and validate restriction sites flanking your insert for easy, directional sub-cloning
• Kanamycin and ampicillin resistance for your choice of selection in E. coli
• Easy blue⁄white colony screening for selection of recombinants
• Includes OneShot® TOP10 Competent cells for added convenience and highest cloning efficiency