BNIP3 Antibody

BNIP3 Antibody (catalog# NBP1-77683)

Novus Biologicals announced today a new BNIP3 Antibody (NBP1-77683) for use as a mitochondrial marker and in transcription regulation research.

BCL2/adenovirus E1B 19 kd-interacting protein 3 (BNIP3) is a mitochondrial dimer protein responsible for protecting cells from viral-induced cell death. The BNIP3 protein also acts as a pro-apoptotic factor due to its BH3 domain. Our new BNIP3 specific antibody will serve as a useful mitochondrial marker, as well as a tool for viral resistance and apoptosis research.

Novus’ new BNIP3 Antibody (NBP1-77683) has been validated for use in Western blot and immunostaining on human samples. However, we also offer an Innovator’s Reward™ Program for testing in novel species and applications.

IHC Image of BNIP3

IF Image of BNIP3

IHC analysis of mouse kidney using BNIP3 antibody
(NBP1-77683) with DAB visualization and a hematoxylin counterstain.

Immunocytochemistry: BNIP3 Antibody [NBP1-77683] - BNIP3 antibody was tested IF analysis of HeLa cells using BNIP3 antibody (NBP1-77683) with a FITC secondary (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

 

BNIP3 In The News

1.     Naushad SM, Prayaga A, Digumarti RR, Gottumukkala SR, Kutala VK. Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 (BNIP3) expression is epigenetically regulated by one-carbon metabolism in invasive duct cell carcinoma of breast. Mol Cell Biochem. 2011 Oct 11. [PMID:21987236]

2.     Liu F, Liu Q, Yang D, Bollag WB, Robertson K, Wu P, Liu K. Verticillin a overcomes apoptosis resistance in human colon carcinoma through DNA methylation-dependent upregulation of BNIP3. Cancer Res. 2011 Nov 1;71(21):6807-16. [PMID:21911457]

3.     Lee Y, Lee HY, Hanna RA, Gustafsson AB. Mitochondrial autophagy by Bnip3 involves Drp1-mediated mitochondrial fission and recruitment of Parkin in cardiac myocytes.Am J Physiol Heart Circ Physiol. 2011 Nov;301(5):H1924-31. [PMID: 21890690]

4.     Gordon JW, Hai Y, Dhingra R, Gang H, Kirshenbaum LA. 28 Preferential targeting of BNIP3 isoforms to mitochondria and endoplasmic reticulum during metabolic stress. Heart. 2011 Dec;97(24):e8. [PMID: 22116916].