Lubio Science, your partner for life science products in switzerland

Molecular Probes® Alkaline Phosphatase Live Stain

2/16/12 5:02 PM

New non-toxic AP live stain maintains viability

Learn how to Identify colonies without killing cells with the new Molecular Probes® Alkaline Phosphatase Live Stain.

This product offers a simple, easy, efficient method to:

•	Identify pluripotent stem cell colonies
•	Preserve cell viability
•	Further propagate identified colonies

Learn how »

If you have any further questions please contact us at info@lubio.ch

Product	List Price	Cat. No.
Alkaline Phosphatase, 50 ul	232.00 CHF	A14353

Posted in New and Featured Products By LuBioScience

Fibronectin from YO Proteins

2/7/12 9:29 AM



We are offering 20% special discount on item no. 663-20MG

Description Fibronectin purified from human plasma (lyophilized) 4 vials 5 mg each Source Human plasma. Donors tested negative for HBsAg, HCV, HIV1 and HIV2 antibodies Purification Affinity chromatography on gelatine agarose and heparin agarose Format Lyophilized from 4x2.5 ml of buffer (150 mM NaCl, 10 mM sodium phosphate pH 7.5). Non-sterile. Reconstitution Add 4x5 ml of warm (37°C) PBS . Incubate at 37°C for 3 hours, occasionally inverting the vial. Do not vortex. Reconstituted solution contains 4x5 mg of soluble fibronectin and a minor quantity of insoluble fibronectin aggregate. Filter slowly through 0.2 µm membrane filter. Concentration 1 mg/ml of fibronectin corresponds to A280 - 1.3

Simply state "YO Fibronectin" when ordering!

* This offer is valid until 31st of December 2012 and cannot be cumulated with any other offer or promotion.

Posted in New and Featured Products Special Offers By LuBioScience

Magnetofectamine by OZ Biosciences

1/31/12 9:06 AM



Magnetofectamine™
The winning combination for optimal transfection efficiency

Ideal to transfect primary and Hard-to-transfect cells, Magnetofectamine™ associates Lipofectamine™ 2000 and OZ Biosciences's CombiMag reagent, increasing the overall efficiency of your transfection while minimizing cytotoxicity. Lipofectamine™ 2000 is the leading transfection reagent on the market for simple and effective nucleic acid delivery (DNA and siRNA). CombiMag is a Magnetofection™ based reagent: versatile and improving transfection efficiency without toxicity. Magnetofection™ is a creative, simple and highly efficient method to transfect cells in vitro and in vivo. This magnetic assisted transfection is based on the use of magnetic nanoparticles associated to the nucleic acid. In this way, the magnetic force allows a rapid concentration of genetic material onto cells and promotes cellular uptake. - Superior transfection efficiency - Enhanced Lipofectamine™ 2000 efficiency - Ideal for hard-to-transfect and primary cells - Less nucleic acids used - minimized toxicity - Serum compatible and applicable to all nucleic acids - Simple, ready-to use and rapid - No need to change your standard Lipofectamine™ 2000 protocol
For more information & results please click here For promotion please click here

Posted in News New and Featured Products By LuBioScience

Novus' Spotlight on LDL & VLDL Receptors

1/31/12 9:05 AM

Spotlight on LDL and VLDL Receptors

Low density lipoprotein (LDL) and very low density lipoprotein (VLDL) receptors are cell-surface receptor proteins that regulate endocytosis of cholesterol and lipoproteins into mammalian cells. These receptors are essential for proper lipid metabolism and healthy cell function. However, LDLR and VLDLR are also associated with a wide variety of diseases, due to their role as gateways between external plasma and cells.
A 2011 study in Hepatology analyzed the role of the LDLR in the Hepatitis C virus life cycle, and found that the physiological function of LDLR is necessary for optimal replication of the Hepatitis C virus genome. Mutations in the LDLR gene cause the autosomal dominant disorder,hypercholesterolemia. Alternatively, defects in VLDLR can cause cerebellar ataxia, mental retardation and dysequilibrium syndrome type 1. VLDLR may also be a susceptibility gene for Alzheimer diseases due to the Reelin binding that induces modulation of Tau phosphorylation.
Novus offers a number of top quality LDLR and VLDLR antibodies for lipid and metabolism research, some of which are featured below.

Featured LDLR and VLDRL Antibodies

VLDL Receptor Antibody (6A6) Mouse Monoclonal Catalog #: NBP1-78162 Sample Size Available Reacts with: Bv, Hu, Mu, Rt Works in: ICC, WB, IF, IHC-P	LDL Receptor Antibody (C7) Mouse Monoclonal Catalog #: NBP1-78159 Sample Size Available Reacts with: Bv, Hu, Mu, Rt Works in: ICC, WB, IF, IP, IHC-P

Immunohistochemical analysis of VLDL Receptor in mouse kidney using NBP1-78162.	ICC analysis of LDL Receptor antibodytested in HepG2 cells with FITC (green). Nuclei were counterstained with Dapi (blue) using NBP1-78159.

New DyLight Antibodies and Conjugation Kits

Novus is proud to offer a wide variety of our antibodies in DyLight conjugated formats. Additionally, Lightning Link conjugation kits are now available for the spectra of DyLight dyes.

Posted in New and Featured Products By LuBioScience

Novus' Featured RUNX2 Antibody

1/31/12 8:59 AM

Featured RUNX2 Antibody

RUNX2 Antibodies (catalog# NBP1-77461 & NBP1-77462)

Novus Biologicals announced today two new RUNX2 Antibodies (NBP1-77461 & NBP1-77462) for use in research on osteoblast differentiation and bone formation.

Runt-related transcription factor 2 (RUNX2) is a transcription factor important for osteoblast differentiation and maturation, as well as skeletal morphogenesis. Novus’ new RUNX2 Antibodies (NBP1-77461 & NBP1-77462) have been validated for use in immunofluorescence staining of human and mouse samples. However, we also offer an Innovator’s Reward™ Program for testing in novel species and applications.


Immunofluorescece staining of HeLa cells using RUNX2 Antibody (NBP1-77461) with a FITC (green) secondary. Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).	Immunohistochemistry staining of mouse prostate tissue using RUNX2 Antibody (NBP1-77462) with DAB visualization and a hematoxylin counterstain.

RUNX2 in the News:

1. van der Deen M, Akech J, Lapointe D, et al. Genomic promoter occupancy of runt-related transcription factor RUNX2 in osteosarcoma cells identifies genes involved in cell adhesion and motility. J Biol Chem. 2011 Dec 9. [PMID: 22158627]

2. Tu X, Joeng KS, Long F. Indian hedgehog requires additional effectors besides Runx2 to induce osteoblast differentiation. Dev Biol. 2011 Dec 2. [PMID: 22155527]

Novus also offers other RUNX2 antibodies, recombinant proteins and control lysates, as well as other ancillary reagents.

Posted in New and Featured Products By LuBioScience

Novus' Featured Cytokeratin 19 Antibodies

1/31/12 8:47 AM

Featured Cytokeratin 19 Antibodies

Cytokeratin 19 N-term Antibody (NBP1-78278)
Cytokeratin 19 C-term Antibody (NB100-687)

Novus Biologicals offers two excellent Cytokeratin 19 Antibodies (NB100-687 & NBP1-78278) for use in cancer research and cytoskeleton studies.

Cytokeratin 19 is an intermediate filament Keratin protein found in epithelial cells, and an important biomarker for lung, breast, lymph node, peripheral blood, and bone marrow tumors. Novus’ new Cytokeratin 19 Antibodies (NB100-687 & NBP1-78278) have been validated for use in immunofluorescence staining and Western blot on human and mouse samples. However, we also offer an Innovator’s Reward™ Program for testing in novel species and applications.


Western blot analysis of Cytokeratin 19 in MCF7 lysate using NBP1-78278.	Immunohistochemistry analysis of Cytokeratin 19 in human kidney carcinoma using NBP1-78278 with DAB visualization and a hematoxylin counterstain.

Immunocytochemistry analysis of Cytokeratin 19 in MCF7 cells using NB100-687 (1:25) with a FITC (green) secondary. Nuclei were counterstained with Dapi (blue).

Western Blot analysis of Cytokeratin 19 using NB100-687 in 1.HepG2 and 2.MCF7 lysates.

Cytokeratin 19 in the News:

1. Terada T. Primary clear cell adenocarcinoma of the lung. Med Oncol. 2011 Dec;28Suppl 1:593-5. [PMID: 21082293].

2. Martínez-Conesa EM, Espel E, Reina M, Casaroli-Marano RP. Characterization of ocular surface epithelial and progenitor cell markers in human adipose stromal cells derived from lipoaspirates. Invest Ophthalmol Vis Sci. 2011 Dec 22. [PMID: 22199247]

Posted in New and Featured Products By LuBioScience

The FLoid™ Cell Imaging Station - Click. Print. Love.

1/30/12 11:42 AM

Click. Click. Print. Love. Go from sample to high-quality image in just a few clicks

Yes, the FLoid™ Cell Imaging Station is that easy

Designed in collaboration with fluorescence microscopy users, the FLoid™ Cell Imaging Station captures high-quality, three-color fluorescent cell images right at your benchtop, with an interface that is so simple even novice users can collect data in just a few clicks of the mouse.

Experience the simplicity of the new FLoid™ Cell Imaging Station for yourself. Test drive the interactive user interface today.


•	Simple
•	Practical
•	Intuitive
•	Informative

Request a demo>

Floid™ Cell Imaging Station - Demo Video

For further information please download the FLoid™ brochure or send an email to info@lubio.ch

Posted in New and Featured Products By LuBioScience

Latest News from Cell Biolabs

1/23/12 5:01 PM

Cell Biolabs, Inc.

Novel AAV Helper Free

Systems

In This Issue

AAV Helper Free Systems

TECH CORNER: ViraBind AAV Purification Kit

Measuring AAV Titer

AAV Purification

Increase Transduction Efficiency

New 2012-2013 Catalog will contain many new cutting-edge products.

Pre-order your copy: info@lubio.ch

Other Cell Biolabs Product Lines

Cell-Based Assays

Cell Signaling & Protein Biology

Infectious Disease Research

Metabolism Research

miRNA Analysis

Oxidative Stress/Damage

Stem Cell Research

Viral Expression

Recombinant AAV has become a useful tool for gene delivery into a wide variety of target cells, because it can transduce non-dividing cells and generates an extremely low immune response.

Read on to find out how our broad selection of AAV kits and reagents can improve your gene expression studies.

AAV Helper Free Systems Increase Infectivity

The AAV Helper Free System was developed to make production of AAV safer Recombinant AAV Virus

and more convenient. We have expanded the AAV Helper Free System to create the broadest portfolio of AAV expression systems.

Our AAV Helper Free Systems are available in the following formats:

AAV native serotypes 1 through 6
AAV-DJ, engineered by DNA family shuffling to create a hybrid capsid from 8 AAV serotypes; the resulting vector conveys significantly higher infectivity rates compared to native AAV across a broad range of cells and tissue types
AAV-DJ/8, a mutant of AAV-DJ that exhibits increased uptake in brain tissue in vivo, similar to AAV-8 and AAV-9
Each Helper Free Expression System includes your choice of 7 different expression vectors including our newest AAV promoterless vector

For more information please contact us

Measure AAV Titer Quickly and Easily

AAV-GFP Quantitation of AAV has traditionally been done by DNA dot blots, which can be time-consuming and suffer from high inter-assay variability. Our QuickTiter™ AAV Quantitation Kit quantifies the nucleic acid from AAV particles in less than two hours. The kit uses proprietary technology to quantify unpurified AAV-2 or AAV-DJ supernatant and purified AAV of any serotype.

For more information please contact us

Purify AAV Without an Ultracentrifuge!

ViraDuctin Kit

Ultracentrifugation is a tedious, time-consuming way to concentrate
AAV, and it only provides partial purification.

Our ViraBind™ AAV Purification Kits can purify your AAV-2 or AAV-DJ preps without the use of an ultracentrifuge.

For more information please contact us

Maximize AAV Transduction Efficiency

AAV infection of your target cell involves a rate-limiting second-strand synthesis step, which can limit transduction efficiencies leading to poor gene expression.

The ViraDuctin™ AAV Transduction Kit is a multi-reagent formulation that can increase transduction efficiency of AAV vectors.

For more information please contact us: info@lubio.ch

Posted in New and Featured Products By LuBioScience

New small volume Lipofectamine™ reagents

1/16/12 3:59 PM

With over 30'000 citations and numeros applications, the Lipofectamine^TM family of transfection reagent has become the laboratory standard for transfection.


•	Outstanding transfection efficiency and viability
•	Exeptional ease of use
•	One of the best and most reliable tranfection reagents for 10 years

Discover the new Lipofectamine™ pack sizes for added convenience: the most cited transfection reagent now all come in 0.3 ml.

Product	Size	Cat. No.
Lipofectamine™ Transfection Reagent	0.3 ml	18324010
Lipofectamine™ Transfection 2000 Reagent	0.3 ml	11668030
Lipofectamine™ LTX Transfection Reagent	0.3 ml	15338030
Lipofectamine™ RNAiMAX Transfection Reagent	0.3 ml	13778030

Posted in New and Featured Products By LuBioScience

PrestoBlue™ - the Reliable Cell Viability Reagent from Life Technologies

1/16/12 3:07 PM

PrestoBlue^TM Cell Viability Reagent

→ Save time with an incubation step as short as 10 minutes
→ Obtain high-quality results with large assay window and Z' values
→ Experience convenience with an add-and-read homogenous format
→ Monitor living cells and perform downstream functional assays with no cell lysis

If you are using alamarBlue^®, MTT or CellTiter-Blue^®, now is the time to try PrestoBlue^TM Cell Viability Reagent

Product	Size	Cat. No.
PrestoBlue™ Cell Viability Reagent	25 ml	A13261
PrestoBlue™ Cell Viability Reagent	100 mL	A13262

Download brochure >

If you have any questions on the product send us an email to info@lubio.ch!

Posted in New and Featured Products By LuBioScience

Introducing the unique Gibco® peel-off sticker

1/16/12 1:08 PM

Peel-Off & Stick On Your Lab Note-Book or Media Bottles

We know the lab is a busy place and things can happen, sometimes beyond your control. Even the most meticulous of you may find yourselves looking for some information that’s not handy, or a sticky note that had your scribbled notes, now seems to be missing! Introducing the unique peel off sticker on Gibco® media, sera and reagents bottles.

Designed to help you easily record information about your media components for better control of your experiments.

Handy reference for lot number and expiration date
Easy re-ordering (product name and catalog #)
Space for a short personal note

The new peel-off sticker is easy to peel-off and stick on your lab note-book next to your experimental data, or on the bottle or flask where you mix your complete media. You should start seeing the new peel-off stickers on your bottle labels in the near future as you order new supplies of media, sera and reagents. Meanwhile, here’s a chance for you to participate in the...

Gibco® Peel-Off Instant Win Game

Enter the numbers from your peel-off label and you could be an instant winner*.

Play the Gibco® instant win game >

Posted in New and Featured Products By LuBioScience

Spotlight on CASP8 and STAG2

1/5/12 11:29 AM

Spotlight on CASP8 and STAG2

Malignant melanoma, the most dangerous type of skin cancer, is estimated to affect roughly one in every 65 adults. Although it is typically preventable, the only treatment is surgical tumor excision. Unfortunately, it is often difficult to prevent the cancer from spreading due to melanoma's high malignancy rates.

Scientists at the University of Leeds recently found that a DNA defect in the apoptosis mediator gene CASP8 significantly increases the risk of melanoma [PMID: 21983787]. Additionally, researchers at the Georgetown University School of Medicine discovered that one fifth of melanomas have a defective copy of the STAG2 Gene, which plays a role in the division of genetic material between cells. [PMID: 2021852505]. These exciting new discoveries offer promise for potential future treatments, and open important new avenues for melanoma research.

Here at Novus Biologicals we offer a number of CASP8 and STAG2 Antibodies, a few of which can be found below:

Caspase 8 Antibody Rabbit Polyclonal Catalog # NB600-576 Reacts with: Bv, Hu, Mu, Rt, Mk Works In: IHC-P, IP, WB	STAG2 Antibody Goat Polyclonal Catalog # NB100-300 Reacts with: Hu, Xp Works In: ICC, WB

Formalin-fixed, paraffin-embedded human stomach stained with Caspase 8.	Nuclear extract from ~500,000 HeLa cells. Antibody: Affinity purified goat anti-SA2 used at 0.5 mcg/ml. Detection: Chemiluminescence with 15 sec. exposure.

New Cancer Antibodies

Laminin Protein Catalog # NBP1-78301 Reacts with: Mu Works In: Func, TCS	Survivin Antibody Mouse Monolconal Catalog # NB500-644 Reacts with: Hu, Rt Works In: ICC, IF, IHC-P

Schwann cells on Laminin-1 substratum	Survivin 8E2 antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with Dapi (blue).

Posted in New and Featured Products By LuBioScience

Six New Apoptosis Antibodies from Novus

12/22/11 8:26 AM

Six New Apoptosis Antibodies

Apoptosis is a critical response to cellular stress or damage, necessary for the proper development and maintenance of any multicellular organism. Over the past 20 years, apoptotic regulation has increasingly become an important area of study for everything from stem cell differentiation to cancer suppression.

Novus is committed to supplying top quality reagents for apoptosis studies. Therefore, we are proud to announce the release of six new apoptosis related antibodies for Western blot analysis and immunostaining procedures.

Survivin Antibody (catalog# NB500-644)
Notch 1 Antibody (catalog# NBP1-78292)
IGF1R Antibodies (catalog# NBP1-77679 & NBP1-77680)
BNIP3L Antibody (catalog# NBP1-78264)
TGF beta 1 Antibody (catalog# NBP1-78398)


Immunofluorescence stain of HeLa cells using Survivin 8E2 antibody (NB500-644) with a FITC (green) secondary. Nuclei were counterstained with Dapi (blue).	Immunohistochemistry stain of human kidney carcinoma tissue using Notch1 Antibody (NBP1-78292)with DAB visualization and ahematoxylin counterstain.	Immunofluorescence stain of A431 cells using IGF1 Receptor Antibody (NBP1-77679) with a FITC (green) secondary. Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).


Western Blot analysis of 1) HeLa, 2) HIH/3T3, 3) SK-BR-3, and 4) COS7 cell lysates using IGF1 Receptor Antibody (NBP1-77680).	Immunohistochemistry stain of mouse prostate tissue using BNIP3L Antibody (NBP1-78264) with DAB visualization and ahematoxylin counterstain.	Immunofluorescence stain of HepG2 cells using TGF beta 1 antibody (NBP1-78398) with a FITC(green) secondary. Nuclei were counterstained with Dapi (blue).

Novus also offers other apoptosis related antibodies, recombinant proteins, control lysates, and RNAi. Contact us (info@lubio.ch) to receive an apoptosis brochure or any other brochure from Novus Biologicals, Inc.

Posted in New and Featured Products By LuBioScience

Novus' Spotlight on Beclin 1

12/20/11 12:00 PM

Spotlight on Beclin 1

Beclin 1 is a 60kDa, BH3-only domain autophagy protein that is essential for the regulation of macroautophagy and the formation of the autophagic vacuole. Through a diverse network of protein interactions, Beclin 1 appears to be a key player in the regulation of a number of critical cellular processes. Specifically, it is a key component of the class III PI3K complex, and interacts with the anti-apoptotic protein, BcL2. Beclin 1 contains 3 identical structural domains comprised of an N-terminal BH3 domain, a central coiled-coil domain and an evolutionarily conserved domain. The expression of Beclin 1 appears to be generally ubiquitous in human and mouse tissues and it is localized predominantly within cytoplasmic structures such as the mitochondria, ER and the perinuclear membrane. Novus offers more than 30 Beclin 1 antibodies for a wide variety of species and applications, which serve as useful tools for autophagy research.

Beclin 1 Antibodies

Beclin 1 Antibody Rabbit Polyclonal Catalog # NB500-249 Reacts with: Hu, Mu Works in: ICC, IF, IHC, IHC-P, IP, WB	Beclin 1 Antibody Mouse Monoclonal Catalog # NBP1-00085 Reacts with: Bv, Ch, Eq, Hu, Mk, Mu, Po, Rt Works in: IHC-P, WB	Beclin 1 Antibody Rabbit Polyclonal Catalog # NBP1-59003 Reacts with: Hu Works in: WB

IHC analysis of Beclin 1 in pheochromocytes of the adrenal medulla using NB500-249.	IHC analysis of Beclin 1 in mouse lung using NBP1-00085.	WB analysis of Beclin 1 in MCF7 tissue lysate using NBP1-59003 at a concentration of 1ug/mL

Other Beclin 1 Antibodies

Beclin 1 Lysate
Catalog # NBL1-07966
Reacts with: Hu
Works in: WB

WB Analysis of Beclin 1 using NBL1-07966
Left lane: control, Right lane:
Over-expression Beclin 1 lysate

Posted in New and Featured Products By LuBioScience

Oxford Biomedicals TBARS' News

12/20/11 11:12 AM

Oxford Biomedical Research has recently redesigned its FR40 TBARS assay to increase sensitivity and reduce interferences. We have also introduced a fluorometric TBARS assay with exceptional sensitivity. These kits have the best sensitivity and the least interference of all kits commercially available. While all TBARS assays detect endogenous interfering factors, many more are generated when samples are boiled with strong acid. Their new, patent pending method uses a milder acid and can be run at room temperature.

Cat. No.	Description	Listprice in CHF
FR35	Color/Fluoro Cuvette TBARS	453.00
FR40	Colorimetric Microplate TBARS	290.00
FR45	Fluorometric Microplate TBARS	314.00

Posted in New and Featured Products By LuBioScience

Novus' XBP1 Antibody

12/19/11 3:23 PM

XBP1 Antibody

XBP1 Antibody (catalog# NBP1-77681)

Novus Biologicals announced today a new XBP1 Antibody (NBP1-77681) for use in immune response and endoplasmic reticulum stress research.

X-box binding protein 1 (XBP1) is a nuclear transcription factor which activates the unfolded protein response during endoplasmic reticulum stress response. Specifically, XBP-1 is upregulated during ER stress, and directly binds the unfolded protein response element to induce mis-folding of several downstream proteins.

XBP-1 is also essential for the growth and differentiation of hepatocyte and plasma cells, as well as some brain development and cognitive ability formation. It has been implicated in a wide range of disorders, including Alzheimer’s disease, major affective disorder type 7, Crohn’s disease and IBS.

Novus’ new XBP1 Antibody (NBP1-77681) has been validated for use in immunofluorescence staining of human and mouse samples. However, we also offer an Innovator’s Reward™ Program for testing in novel species and applications.

Immunoshitochemistry stain in mouse bladder using XBP1 antibody with DAB visualization and ahematoxylin counterstain.

Immunofluorescence stain in HeLa cells using XBP1antibody with a FITC (green) secondary. Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

XBP1 In The News

1. Iwawaki T, et al. Function of yeast and amphioxus tRNA ligase in IRE1alpha-dependent XBP1 mRNA splicing.BiochemBiophys Res Commun, 2011 Oct 7. PMID 21924241.

2. Bae J, et al. Identification of novel myeloma-specific XBP1 peptides able to generate cytotoxic T lymphocytes: a potential therapeutic application in multiple myeloma.Leukemia, 2011 Oct. PMID 21660045.

3. Dong L, et al. Ets-1 mediates upregulation of Mcl-1 downstream of XBP-1 in human melanoma cells upon ER stress. Oncogene, 2011 Aug 25. PMID 21423203.

We are also offering other XBP1 antibodies and recombinant proteins, control lysates and RNAi as well asother ancillary reagents from Novus Biologicals.

Posted in New and Featured Products By LuBioScience

Novus' BNIP3 Antibody

12/19/11 3:08 PM

BNIP3 Antibody

BNIP3 Antibody (catalog# NBP1-77683)

Novus Biologicals announced today a new BNIP3 Antibody (NBP1-77683) for use as a mitochondrial marker and in transcription regulation research.

BCL2/adenovirus E1B 19 kd-interacting protein 3 (BNIP3) is a mitochondrial dimer protein responsible for protecting cells from viral-induced cell death. The BNIP3 protein also acts as a pro-apoptotic factor due to its BH3 domain. Our new BNIP3 specific antibody will serve as a useful mitochondrial marker, as well as a tool for viral resistance and apoptosis research.

Novus’ new BNIP3 Antibody (NBP1-77683) has been validated for use in Western blot and immunostaining on human samples. However, we also offer an Innovator’s Reward™ Program for testing in novel species and applications.


IHC analysis of mouse kidney using BNIP3 antibody (NBP1-77683) with DAB visualization and a hematoxylin counterstain.	Immunocytochemistry: BNIP3 Antibody [NBP1-77683] - BNIP3 antibody was tested IF analysis of HeLa cells using BNIP3 antibody (NBP1-77683) with a FITC secondary (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

BNIP3 In The News

1. Naushad SM, Prayaga A, Digumarti RR, Gottumukkala SR, Kutala VK. Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 (BNIP3) expression is epigenetically regulated by one-carbon metabolism in invasive duct cell carcinoma of breast. Mol Cell Biochem. 2011 Oct 11. [PMID:21987236]

2. Liu F, Liu Q, Yang D, Bollag WB, Robertson K, Wu P, Liu K. Verticillin a overcomes apoptosis resistance in human colon carcinoma through DNA methylation-dependent upregulation of BNIP3. Cancer Res. 2011 Nov 1;71(21):6807-16. [PMID:21911457]

3. Lee Y, Lee HY, Hanna RA, Gustafsson AB. Mitochondrial autophagy by Bnip3 involves Drp1-mediated mitochondrial fission and recruitment of Parkin in cardiac myocytes.Am J Physiol Heart Circ Physiol. 2011 Nov;301(5):H1924-31. [PMID: 21890690]

4. Gordon JW, Hai Y, Dhingra R, Gang H, Kirshenbaum LA. 28 Preferential targeting of BNIP3 isoforms to mitochondria and endoplasmic reticulum during metabolic stress. Heart. 2011 Dec;97(24):e8. [PMID: 22116916].

Posted in New and Featured Products By LuBioScience

Novus' Spotlight on Jumonji

12/19/11 2:09 PM

Spotlight on Jumonji

Jumonji, Japanese for cruciform, was originally named for the appearance of the neural plates in jumonji mutant nice, and means cruciform in Japanese. Within the protein, jumonji contains an AT-rich interacting domain, a jmjN domain, and a jmjC domain. Since the discovery of this gene, jmjC domains have been found in over 100 other proteins, and are evolutionarily conserved from human to yeast. JmjC domains play a role in stem cell differentiation and renewal, tumor suppression, obesity resistance, and gene expression.

Recently, some jumonji family members have been identified as histone demethylases. Jumonji member JMJD1A specifically demethylates mono and dimethyl forms of histone 3 Lysine 9. Another member, JMJD2A also works specifically on H3 Lys9, but in the trimethylated form, along with trimethyl H3 Lys36. JMJD2C also works on these sites, but is specific for the trimethyl forms.

Featured Jumonji Antibodies

Immunofluorescence staining in HeLa cells using JHDM2A antibody (NBP1-49601) at 1:50 with a FITC (green) secondary. Nuclei were counterstained with DAPI (blue).

Immunohistochemical staining of paraffin embedded mouse pancreas using JMJD2A antibody (NBP1-49602).


Immunohistochemical staining of paraffin embedded mouse pancreas using JMJD2C antibody (NBP1-49600).	WB analysis of HepG2 cell lysate using JMJD3 antibody (NBP1-06640).

EpiPlus Antibodies

Novus' EpiPlus™ antibodies are specific for modified histone residues, allowing researchers to monitor changes in specific histone states. The EpiPlusTM line of antibodies has been extensively characterized and validated in ChIP, Western blot, and immunostaining on human, mouse, and rat samples. TheEpiPlusTM Antibodies are therefore extremely valuable tools for epigenetics research and will facilitate research on epigenetic regulation.

Epigenetics Literature and Poster

Novus offers complimentary epigenetics and Epi-Plus™ product fliers, as well as a Histone Modification Map Poster and many other catalogs and posters. For more information please contact us on info@lubio.ch

Posted in New and Featured Products By LuBioScience

New TP53INP2 & GABARAP Antibodies from Novus Biologicals

12/19/11 1:47 PM

New TP53INP2 & GABARAP Antibodies

TP53INP2 antibody (catalog# NBP1-71704) & GABARAP antibody (catalog# NBP1-71771)

Novus Biologicals announced new antibodies to TP53INP2 (NBP1-71704) and GABARAP
(NBP1-71771) for use in autophagy research.

Tumor Protein 53-Induced Nuclear Protein 2 (TP53INP2) is thought to be a scaffold protein which translocates from the nucleus to autophagosomes during preparation for autophagy. TP53INP2 binds LC3-related proteins, including GABARAP and VMP1. Research has shown that both TP53INP2 and GABARAP are required for autophagy in mammalian cells.

Immunofluorescence analysis of TP53INP2 in HeLa cells using NBP1-71704 at 1:200 with a FITC secondary (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

Immunohistochemistry analysis of TP53INP2 in mouse prostate using NBP1-71704.


Immunohistochemistry analysis of GABARAP in mouse liver using NBP1-71771.	Immunofluorescence analysis of GABARAP in HeLa cells using NBP1-71771 at 1:200 with a FITC secondary (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

These new antibodies have been validated for use in immunofluorescence staining on human and mouse samples. However, we also offer an Innovator’s Reward™ Program for testing in novel species and applications.

TP53INP2 in the News:

1. Nowak J, Archange C, Tardivel-Lacombe J, et al. The TP53INP2 protein is required for autophagy in mammalian cells. MolBiol Cell. 2009 Feb;20(3):870-81. [PMID: 19056683].

Posted in New and Featured Products By LuBioScience

Invitrogen's video "Ph Diddy is on the scene" - Life in the lab

12/8/11 8:51 AM

Ph. Diddy's story

Arnold Young, (aka the Ph.Diddy) is a biotech Ph.D candidate. Arnold was an A+ student, and is now ready to take over the world of science! He soon discovers how life in the lab is filled with drive and devotion, frustrations and fulfillment, hard work, late hours, repeated experiments, peer review and a strive for respect and recognition. How will the ups and downs of life in the lab shape our Ph.Diddy on his journey to have his first scientific paper published?

Acknowledgements: This video is inspired by all the dedicated everyday-heroes-of-science. Invitrogen recognizes your passion and admires your perseverance!

While watching this video download your free copies of BenchTop Devices brochure, Immunology Catalog or Molecular Biology Essentials catalog to see everything that is going on at Life Technologies!

If you have any further questions on the prodcuts please contact us at info@lubio.ch

BY POPULAR DEMAND - the song is now available on iTunes! http://itunes.apple.com/us/album/the-ph.diddy-is-on-the-scene/id478987896?i=4...

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Novus' Spotlight on Pancreatic Cancer Stem Cells

11/30/11 2:33 PM

Spotlight on Pancreatic Cancer Stem Cells

A recent study done at the Spanish National Cancer Research Center (CNIO), published in the November issue of Cell Stem Cell [PMID:22056140] , successfully demonstrates a therapeutic strategy for targeting pancreatic cancer stem cells (CSC). Their research shows that the Nodal/Activin pathway is critical for the self-renewal capacity and the stemness properties of pancreatic CSC. In addition, Nodal/Activin is strongly expressed by pancreatic CSC, and also by pancreatic stellate cells (PSC). PSC are abundant in the stroma that surrounds the pancreatic CSC, and may potentially be the supportive niche for these CSC. They demonstrated that by targeting the stroma as well as the CSC they were able to effectively treat pancreatic cancer in a number of preclinical models.

Featured Nodal Antibody

Nodal Antibody
Rabbit Polyclonal
Catalog #: NBP1-71686
Reacts with: Hu, Mu, Rt
Works in: ICC, IF, WB

ICC staining of NODAL in HeLa cells using NBP1-71686.

Featured Stem Cell Marker Antibodies

OCT4 Antibody - Embryonic Stem Cell Marker Rabbit Polyclonal Catalog #: NB100-2379 Sample Size Available Reacts with: Bv, Fe, Hu, Mk, Mu, Po Works in: FACS, ICC, IF, WB	Cripto1 Antibody Rabbit Polyclonal Catalog #: NB100-1597 Sample Size Available Reacts with: Hu Works in: FACS, WB	Nanog Antibody - Embryonic Stem Cell Marker Rabbit Polyclonal Catalog #: NB100-58842 Sample Size Available Reacts with: Mu Works in: ICC, IHC-P

FACS staining of NTERA-2 cells using NB100-2379 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.	Western blot analysis of Cripto in MDA-MB231 lysates.	IHC staining of Nanog in paraffin embedded mouse testis.

Posted in New and Featured Products By LuBioScience

Novus' Heat Shock Protein News

11/23/11 11:16 AM

Spotlight on Heat Shock Protein 70

Excessive environmental stress induces a complex cellular reaction known as the heat shock response, to protect proteins from damage. A special class of proteins called Heat Shock Proteins (HSP) is significantly upregulated upon cellular stress, such as hypoxia, infection, inflammation, or starvation. Of the three most widely studied HSPs (HSP60, HSP70 & HSP90), HSP70 has begun to emerge as critical factor in the hypoxic stress response mechanism.

Many researchers have shown that HSP70 expression is strongly increased in cells exposed to hypoxic conditions. However, recent studies have revealed more about the functional role that HSP70 plays in the heat shock response and the protective effects that it confers on the cell. See our "HSP70 in the News" section below for more details.

Novus is committed to supporting this groundbreaking research on hypoxia and remains a leading supplier of top quality heat shock protein reagents.

Featured Heat Shock Protein 70 Antibodies

Hsp70 Antibody (NBP1-77455)	Hsp70 Antibody (NBP1-77456)

IF stain of HSP70 in HeLa cells using NBP1-77455 at 1:25 with FITC (green) secondary. Nuclei were counterstained with Dapi (blue).	IHC stain of Hsp70 in human xenograft kidney cancer using NBP1-77456. DAB visualization with hematoxylin counterstain.

HSP70 In The News

1. Zhang K, Zhao T, Huang X, et al. Preinduction of HSP70 promotes hypoxic tolerance and facilitates acclimatization to acute hypobaric hypoxia in mouse brain. Cell Stress Chaperones. 2009 Jul;14(4):407-15. [PMID: 19105051]

2. Wei Z, Bing Z, Jiu L, Xue Y. The protective effect of heat shock protein 70 gene transfection on rats with cerebral hypoxia-ischemia. 2011 Jun;23(6):365-7. [PMID: 21672388].

3. Azad P, Ryu J, Haddad GG.Distinct role of Hsp70 in Drosophila hemocytes during severe hypoxia. Free RadicBiol Med. 2011 Jul 15;51(2):530-8. [PMID: 21616137]

4. Lin HJ, Wang CT, Niu KC, et al. Hypobaric hypoxia preconditioning attenuates acute lung injury during high-altitude exposure in rats via up-regulating heat-shock protein 70. ClinSci (Lond). 2011 Sep;121(5):223-31. [PMID: 21599636]

Additional New Heat Shock Protein Antibodies

HSP27 (NBP1-75477)
HSP60 (NBP1-77396 / NBP1-77397)
HSP90A (NBP1-77682 / NBP1-77685)
HSP90B (NBP1-77561 / NBP1-77563)

Posted in New and Featured Products By LuBioScience

New Cellular Assay Kits for p53 and Histone H3 from Life Technologies!

11/18/11 1:31 PM

new cellular assay kits for p53 and histone H3

Need a high throughput cellular assay to monitor epigenetic modifying enzymes?

The combination of our BacMam delivery system with LanthaScreen® TR-FRET technology offers an HTS-compatible assay platform for measuring post-translational modifications in a variety of cellular backgrounds.

Our BacMam Histone Cellular Assay Kits allow you to choose the protein modification that corresponds to your enzyme of interest in your cell background. For example, look for inhibitors of histone methyl transferases or demethylases by monitoring the methylation site on histone H3 that corresponds to your enzyme of interest.

Fig. 1: Workflow of the BacMam-enabled cellular assay platform using LanthaScreen® TR-FRET technology

Fig. 1: Workflow of the BacMam-enabled cellular assay platform using LanthaScreen® TR-FRET technology

For more information on how we have used this platform to interrogate post translational modifications of p53 and histone H3, download the poster we recently presented at ELRIG: Mac Donald et al.

download poster

If you would like to more details on our extended range of cellular assay kits, please contact us >

Posted in New and Featured Products By LuBioScience

TOP-BLOCK - The perfect blocking solution

6/13/11 9:47 AM

THE PERFECT BLOCKING SOLUTION
FOR VARIOUS APPLICATIONS!





•	Do you have problems with background in Western Blot or ELISA?

•	Do you block with BSA or Serum and spend a lot of money?

Catalog No.	Quantity	Price
TB232010.0200	200 g	189.00 CHF
TB232010.0500	500 g	449.50 CHF
TB232010.1000	1 kg	728.00 CHF

We have the solution!

•		vitrually no background in Western Blotting using phosphorylation specific antibodies
•		increase of signal-to-noise ration in ELISA
•		excellent blocking capacity on different surfaces
•		affordable price

•		higher blocking capacity than BSA or Serum
•		useful in IHC (Immunohistochemistry)
•		gelatine derivative which can be dissolved at RT
•		stays soluble at 4° C in aqueous solutions

Ask for FREE SAMPLES! Contact us at info@lubio.ch

Posted in New and Featured Products By LuBioScience

2/16/12 5:02 PM

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2/7/12 9:29 AM

We are offering 20% special discount on item no. 663-20MG

1/31/12 9:06 AM

The winning combination for optimal transfection efficiency

Ideal to transfect primary and Hard-to-transfect cells, Magnetofectamine™ associates Lipofectamine™ 2000 and OZ Biosciences's CombiMag reagent, increasing the overall efficiency of your transfection while minimizing cytotoxicity.

Lipofectamine™ 2000 is the leading transfection reagent on the market for simple and effective nucleic acid delivery (DNA and siRNA).

CombiMag is a Magnetofection™ based reagent: versatile and improving transfection efficiency without toxicity.

Magnetofection™ is a creative, simple and highly efficient method to transfect cells in vitro and in vivo.

This magnetic assisted transfection is based on the use of magnetic nanoparticles associated to the nucleic acid. In this way, the magnetic force allows a rapid concentration of genetic material onto cells and promotes cellular uptake.

For more information & results please click here

For promotion please click here

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Download brochure >

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Peel-Off & Stick On Your Lab Note-Book or Media Bottles

Gibco® Peel-Off Instant Win Game

Play the Gibco® instant win game >

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